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Research · Dosing · Reference

A precise reference for peptide dosing.

Reported dose, route, timing, frequency, duration and reconstitution for 60 compounds, indexed by research area. Every row names the source that reports it. Select any of them to assemble a schedule you can print or mail to yourself.

60 compounds indexed118 reported protocols25 research areasResearch use only
The line, verbatim

For in-vitro research use only · not for human consumption of any kind. Figures on this page are reproduced from the reference library this site syncs, each row named to the source that reports it. Peppersite does not recommend an amount for any compound, and nothing here has been checked against the studies it derives from.

How to use this reference

Precision matters more than intensity.

More is not better. Peptide signalling is dose-dependent, time-sensitive, and goal-specific, and the same compound at the same amount does different things on different schedules.

What it helps you do
  • Understand typical clinical dosing ranges.
  • Compare timing, frequency, and duration.
  • Avoid common mistakes from overuse or improper escalation.
  • Support thoughtful, conservative application over aggressive protocols.
Always consider
  • Goal: repair, stimulation, replacement, regulation
  • Individual metabolic, nervous-system and hormonal terrain
  • Whether a lighter signal may be sufficient before escalating
60 compounds
CompoundReported doseRouteFrequencyVial · reconstitutionReported by
5-Amino-1MQ
Recovery
Standard: 100 mg once daily · Low: 50 mg once daily · High: 150 mg once dailyoralonce daily8 to 12 weeks on, 4 to 6 weeks offReconstitute each vial with 3.0 mL bacteriostatic (BAC) water for ~16.7 mg/mL; 2 vials = ~6 mL total. (Subcutaneous form; the compound is also taken orally.)
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: 4 to 7 hours. The most common research community target dose is 100 mg once daily.
Advanced Recomp Stack
Body Composition · Metabolic Health · Recovery · Performance
Retatrutide: 2 mg/week (initiation, weeks 1-4) escalating to 12 mg/week (maximum, weeks 17-20+); CJC-1295 (No DAC): 100-200 mcg, 1-2x daily; Ipamorelin: 100-200 mcg, 1-2x dailysubcutaneousRetatrutide: once weekly; CJC-1295 and ipamorelin: 1-2x daily (5-on / 2-off rhythm)Standard cycle 12-16 weeks; Extended cycle 16-24 weeks; Continuous retatrutide + cycled CJC/Ipa (12 weeks on / 4 weeks off). CJC/Ipa cycled 12 weeks on / 4 weeks off; retatrutide can continue.Retatrutide: 5 mg vial + 1 mL BAC water = 5 mg/mL; 10 mg vial + 2 mL BAC water = 5 mg/mL; 10 mg vial + 1 mL BAC water = 10 mg/mL; 20 mg vial + 2 mL BAC water = 10 mg/mL. Volumes over 1.0 mL need a larger syringe or a split injection at two sites. CJC-1295 (No DAC): 2 mg vial + 1 mL BAC water = 2,000 mcg/mL; 5 mg vial + 2 mL BAC water = 2,500 mcg/mL; 5 mg vial + 2.5 mL BAC water = 2,000 mcg/mL. Ipamorelin: same math as CJC-1295. Pre-blended CJC/Ipa combo vial (10 mg = 5 mg CJC + 5 mg ipamorelin): reconstitute with 2 mL BAC water for 2,500 mcg/mL per peptide; 8 units (0.08 mL) = ~200 mcg of each. Label every vial with compound name, concentration, and reconstitution date. Retatrutide is dosed in mg; CJC/Ipa in mcg, a 1,000x difference. Use U-100 insulin syringes.
peptidedosingprotocols.com
What it saysHide(secondary reference; provider confirmation required). [Source: peptidedosingprotocols.com]
AOD-9604
Metabolic Health · Body Composition · Recovery
Standard: 300-500 mcg/day · Low: 300 mcg · High: 500 mcgSubcutaneous injectiondaily12-24 weeks · Cycle context Phase 2 protocols ran 12-24 weeks.3.0 mL bacteriostatic water for a 5 mg vial
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Common research dose is 300-500 mcg/day.
300 mcg SC daily (commonly AM, fasted)subcutaneousonce daily8-12 weeks · 8-12 week cyclesCommonly 2 mg or 5 mg vialsReconstitute with bacteriostatic water
Commonly published practitioner dosing range - provider confirmation required.
250-300 mcgsubcutaneous or oralOnce daily8-12 weeksBacteriostatic water
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Referenced at 250-500 mcg once daily, up to 500 mcg-1 mg for aggressive fat loss protocols. Fasted morning administration cited. Noticeable results reported within 4-6 weeks.subcutaneousOnce dailyMorning, fasted, before eating · 12 weeks on followed by a short break to prevent desensitizationnot stated
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
ARA-290
Recovery
4 mgsubcutaneous or intranasal or oralOnce daily28 daysnot stated
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
BPC-157
Tissue Repair · Soft Tissue Recovery · Tendon/Ligament Recovery · Gut Health · Injury Recovery · Recovery
Standard: 250 mcg twice daily · Low: 250 mcg once daily · High: 500 mcg twice dailySubcutaneous injectionTwice daily4-6 weeks · Standard2 mL BAC water
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: under 30 minutes. The standard dosing for BPC-157 is 250 mcg twice daily.
250-500 mcgsubcutaneous or oral or topicalTwice daily (morning and evening)4-6 weeksBacteriostatic water
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
250-500 mcg once or twice dailysubcutaneousonce or twice daily4-8 weeks · 4-8 week cycles, may repeat after a breakCommonly supplied as 5 mg or 10 mg vialsReconstitute lyophilized powder with bacteriostatic water; refrigerate after mixing
Commonly published practitioner dosing range - provider confirmation required.
Referenced at 200-500 mcg (0.2-0.5 mg) daily overall; recovery contexts cite 200-400 mcg once or twice daily, commonly right after training. Oral form referenced as viable for gut-focused use.subcutaneous, intramuscular near injury, or oral1-2x dailyMorning for systemic or gut focus; post-workout for tissue repair; before bed for overnight healing · On-cycle 4-6 weeks, off-cycle 1-2 monthsnot stated
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
Cagrilintide
Recovery
Standard: 2.4 mg/week · Low: 0.25 mg/week · High: 4.5 mg/weekSubcutaneous injectiononce weekly16 weeksBAC water added per vial; concentration depends on volume chosen.
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: About 159 to 195 hours(~7 to 8 days). Cagrilintide is dosed starting at 0.25 mg once weekly, escalating to 2.4 mg weekly.
Cagrilintide / Retatrutide
Metabolic Health · Body Composition · Weight Management · Appetite Regulation
Retatrutide 12 mg/week + Cagrilintide 2.4 mg/week (maintenance); pre-blend most common as 12.5 mg retatrutide + 2.5 mg cagrilintide per vialSubcutaneousOnce weekly24-week ramp + open-ended maintenance; community: 16-24 weeks on, 4-8 weeks offPre-blend (15 mg vial: 12.5 mg retatrutide + 2.5 mg cagrilintide): add 2.5 mL bacteriostatic water (BAC water) slowly down the vial wall; resulting concentration is 5.0 mg/mL retatrutide + 1.0 mg/mL cagrilintide (6.0 mg/mL total blend). Both compounds use U-100 insulin syringes. BAC water is added slowly down the vial wall. Separate vials each use 1-2 mL BAC water per vial. Reconstituted vials are stable for 28-30 days at 2-8 °C.
peptidedosingprotocols.com
What it saysHide(secondary reference; provider confirmation required). [Source: peptidedosingprotocols.com]
Cagrilintide / Tirzepatide
Metabolic Health · Body Composition · Weight Management · Appetite Regulation
Tirzepatide 15.0 mg/week + Cagrilintide 2.4 mg/week (maintenance); sub-maximal example: tirzepatide 10 mg + cagrilintide 1.2 mgSubcutaneousOnce weekly24-week ramp + open-ended maintenance; or stabilize tirzepatide at 10-15 mg then add cagrilintide for 12+ weeks; or sub-maximal combination as neededEach vial is reconstituted separately using bacteriostatic water (BAC water). Tirzepatide: 5 mg vial + 1.0 mL BAC = 5 mg/mL; 10 mg vial + 2.0 mL BAC = 5 mg/mL; 10 mg vial + 1.0 mL BAC = 10 mg/mL; 30 mg vial + 3.0 mL BAC = 10 mg/mL. Cagrilintide: 5 mg vial + 2.0 mL BAC = 2.5 mg/mL; 10 mg vial + 2.0 mL BAC = 5.0 mg/mL; 10 mg vial + 1.0 mL BAC = 10.0 mg/mL. Cagrilintide is sensitive, inject BAC water down the vial wall and swirl gently; do not shake (amyloid fibril risk); discard cloudy solution. Label every vial with compound name, concentration, and reconstitution date. Both compounds are stable 28-30 days at 2-8 °C once reconstituted. Standard steps: let vial and BAC water reach room temperature (~10-15 minutes); swab both vial stoppers with alcohol. Do not mix the two compounds in one syringe.
peptidedosingprotocols.com
What it saysHide(secondary reference; provider confirmation required). [Source: peptidedosingprotocols.com]
CagriSema
Metabolic Health · Body Composition · Weight Management · Appetite Regulation
cagrilintide 2.4 mg + semaglutide 2.4 mgSubcutaneousOnce weeklyREDEFINE trials ran 68 weeks; research cycles are commonly 12-24 weeks; short investigative cycles 8-12 weeks.1. Wash your hands and lay out a clean work surface. 2. Wipe the rubber stopper of each vial with an alcohol swab and let it dry. 3. Draw the planned BAC water volume into a fresh syringe. 4. Inject the BAC water down the inside wall of the peptide vial, do not spray directly onto the lyophilized powder. 5. Swirl gently until the powder is fully dissolved. Do not shake. 6. Label the vial with the date of reconstitution and the concentration. 7. Refrigerate at 36-46°F (2-8°C) and use within the supplier-specified stability window. For pre-blended vials: BAC water volume is dictated by the vial concentration printed on the COA, there is no universal default for a fixed-dose combination, so verify the supplier's spec before reconstituting. Illustrative examples: 20 mg vial + 2.0 mL BAC water = 10 mg/mL (0.48 mL = 48 units U-100 per weekly maintenance draw); 20 mg vial + 2.5 mL BAC water = 8 mg/mL (0.60 mL = 60 units U-100); 20 mg vial + 3.0 mL BAC water = 6.67 mg/mL (0.72 mL = 72 units U-100). For separate vials: reconstitute each compound independently; e.g. 5 mg vial + 2.0 mL BAC water = 2.5 mg/mL (0.96 mL = 96 units U-100 per 2.4 mg draw); 10 mg vial + 3.0 mL BAC water = 3.33 mg/mL (0.72 mL = 72 units U-100 per 2.4 mg draw).
peptidedosingprotocols.com
What it saysHide(secondary reference; provider confirmation required). [Source: peptidedosingprotocols.com]
CJC-1295
Body Composition · Metabolic Health · Sleep · Recovery · Performance Recovery
100 mcg per injection (1-2 mcg/kg saturation dose)subcutaneous2-3 times daily8-12 weeksBacteriostatic water
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Standard: 200 mcg, once daily · Low: 100 mcg, once daily · High: 300 mcg, up to 3x dailySubcutaneous (SubQ)1-3 times daily8-12 weeks · 4-6 weeks off10 mg vial with 3 mL bacteriostatic water
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: About 30 minutes. The standard dose is 200 mcg, administered once daily.
Non-DAC version referenced at 100-300 mcg (0.1-0.3 mg) per dose due to short half-life. Empty stomach preferred; avoid co-administration with carbohydrates or fats (insulin interference with GH release).subcutaneous1-3x dailyMorning, pre-workout, or before bed · On-cycle 8-12 weeks, off-cycle 6-12 weeksnot stated
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
CJC-1295 (DAC)
Recovery
Standard: 1,000 mcg once weekly · Low: 500-1,000 mcg once weekly · High: 2,000 mcg once weekly or 1 mg twice weeklySubcutaneous injectiononce weekly8-12 weeks · 8-12 weeks on, 4-6 weeks offreconstitute lyophilized powder with bacteriostatic water before use
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: 6-8 days. Common research-planning ranges land between 1-2 mg subcutaneously once weekly.
DAC version referenced at 2-3 mg per dose; extended half-life supports infrequent dosing. Bedtime administration cited to align with sleep-phase GH release.subcutaneous1-2x weeklyOptionally before bed · On-cycle 8-12 weeks, off-cycle 6-12 weeksnot stated
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
CJC-1295 / Ipamorelin (GH Pulse)
Recovery · Body Composition · Longevity · Sleep
200-300 mcg of each peptide (CJC-1295 no DAC and Ipamorelin) daily; lower doses of 100-150 mcg typical for first 2-4 weeks before titrationsubcutaneousOnce dailyStandard: 8-12 weeks on, 4 weeks off; Extended: 16 weeks on, 8 weeks off; Short test: 4-6 weeks on, 4 weeks offFor a 20 mg pre-blended vial (10 mg CJC-1295 + 10 mg Ipamorelin): add 3.0 mL bacteriostatic water for a concentration of 6.67 mg/mL total (3.33 mg/mL each peptide); a 200 mcg dose of each peptide = 6 units (0.06 mL) on a U-100 syringe. Alternative fills: 2.0 mL BAC water = 10.0 mg/mL total (5.0 mg/mL each), 200 mcg dose = 4 units (0.04 mL); 2.5 mL BAC water = 8.0 mg/mL total (4.0 mg/mL each), 200 mcg dose = 5 units (0.05 mL). For separate 10 mg vials: add 3.0 mL BAC water to each = 3.33 mg/mL, 200 mcg dose = 6 units (0.06 mL). Step-by-step: inspect the vial; wipe stopper with alcohol prep pad and let dry; draw planned BAC water volume with a sterile syringe; inject BAC water slowly down the inside wall of the vial (do not spray directly onto powder); gently swirl (do not shake) until powder dissolves; label with date and concentration; refrigerate at 35.6-46.4°F (2-8°C) shielded from light; use within 28-30 days; discard if cloudy, discolored, or contaminated.
peptidedosingprotocols.com
What it saysHide(secondary reference; provider confirmation required). [Source: peptidedosingprotocols.com]
CJC-1295 / Ipamorelin (No Dac)
Recovery
200-300 mcg of each peptide daily, typically administered once daily SubQ.not statednot statedAdd 3.0 mL BAC water to a 20 mg blend vial.
Peptide Dosing Protocols stack
What it saysHide(secondary reference; provider confirmation required).
DSIP
Sleep
Standard: 100-200 mcg · Low: 100 mcg · High: 300 mcgSubQonce daily5-7 nights · short course2.0 mL bacteriostatic water
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). The standard dosing for DSIP is 100-200 mcg subcutaneously once daily.
100-300 mcg subcutaneous; 25 nmol/kg IV (~1.5 mg for 70 kg person)subcutaneous or intranasalOnce daily before bed; or every 2-3 dayshave not been established.Bacteriostatic water
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Epitalon
Longevity · Sleep · Cognitive Support
Standard: 5 mg/day · High: 10 mg/daySubQ2-3 times per year20 days · short cycleAdd 2.0 mL BAC water to a 10 mg vial.
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: half-life of epitalon? Epitalon clears the bloodstream very quickly, on the order of minutes, whic. The standard dosing for Epitalon is 5 mg per day for 20 days.
5-10 mg per daysubcutaneous or oralOnce dailyBacteriostatic water
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Referenced at 10 mg daily for 10 days followed by a break; longevity stacking contexts cite 2-3 cycles per year. General dosage guideline of 5-10 mg per cycle also referenced.subcutaneous or nasalDaily during 10-day cycleEvening cited for sleep-phase cellular repair; morning for circadian alignment · 10-day cycles, 2-3 per yearnot stated
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
FOXO4-DRI
Recovery
Standard: 250 mcg daily · Low: 250 mcg daily · High: 500 mcg dailySubQdaily16 weeks · 4 weeks on, 4 weeks off3.0 mL BAC water
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Standard dosing starts at 250 mcg daily and increases to 500 mcg daily.
GHK-Cu
Tissue Repair · Inflammation · Longevity · Recovery · Post-Procedure Recovery
Standard: 1.0-1.5 mg · Low: 1.0 mg · High: 2.0 mgSubcutaneous injectionDaily4-6 weeks · 4-8 weeks50 mg vial reconstituted with 2.0 mL bacteriostatic water
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). The standard dosing for GHK-Cu is 1.0-1.5 mg daily for 4-6 weeks.
1-2 mg SC daily, or topical applicationsubcutaneous or topicalonce daily30-day cyclesCommonly 50 mg vials (injectable) or topical formulationsReconstitute with bacteriostatic water (injectable)
Commonly published practitioner dosing range - provider confirmation required.
Topical: 0.01-1% in cream/serum; SC injection: 1-2 mg dailysubcutaneous or oral or topicalTopical: once or twice daily; SC: once dailyRepeatableBacteriostatic water
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Topical: serum to face and neck twice daily (AM and PM) after cleansing, before moisturizer. Injectable referenced at 200-300 mcg (0.2-0.3 mg) before bedtime in recovery contexts; sterile technique and supervision stressed. Microneedling before topical cited to enhance absorption.topical or subcutaneousTopical 2x daily; injectable nightly in recovery contextsTopical AM/PM; injectable before bednot stated
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
GHRP-6
Recovery
Standard: 300 mcg · Low: 100 mcg · High: 300 mcgSubQ3x daily, at least 4 hours apartnull · 8 weeks on, 4 weeks off3 mL bacteriostatic water
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: null. GHRP-6 is typically dosed at 100-300 mcg per injection.
Cycling reference: 6-8 weeks on, 4 weeks off. Cited for appetite stimulation and GH release with fat metabolism support.subcutaneousnot statedOn-cycle 6-8 weeks, off-cycle 4 weeksnot stated
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
GLOW
Tissue Repair · Skin & tissue · Soft Tissue Recovery · Recovery
Pre-blend: 0.10 mL (10 units) daily = ~2.33 mg total blend (~1.67 mg GHK-Cu, ~0.33 mg TB-500, ~0.33 mg BPC-157). Separate vials: BPC-157 250-500 mcg/day; TB-500 2.5-5 mg per injection 2x/week (loading), 2 mg per injection (maintenance); GHK-Cu 1-2 mg/day (loading), 1 mg/day (maintenance).subcutaneousOnce daily (pre-blend); separate vials: BPC-157 daily, TB-500 twice weekly, GHK-Cu dailyStandard: 4-6 weeks on, 2-4 weeks off; Extended: 8-12 weeks on, 4-8 weeks off; Skin-focused blend: 4 weeks on, 2-4 weeks off. GHK-Cu copper cycling typically governs cycle length: 30-60 days on, equal time off.Pre-blend (70 mg vial): Add 3.0 mL bacteriostatic water → ~23.3 mg/mL total blend; standard daily dose is 0.10 mL (10 units) = ~2.33 mg total. Separate vials, BPC-157 5 mg vial: add 2 mL BAC water → 2,500 mcg/mL; BPC-157 10 mg vial: add 2 mL BAC water → 5,000 mcg/mL. TB-500 5 mg vial: add 1 mL BAC water → 5 mg/mL; TB-500 10 mg vial: add 2 mL BAC water → 5 mg/mL. GHK-Cu 50 mg vial: add 5 mL BAC water → 10 mg/mL; GHK-Cu 100 mg vial: add 10 mL BAC water → 10 mg/mL. Standard steps: wipe stopper with alcohol swab and let dry; draw planned BAC water volume; aim water at inside of glass wall, not directly onto powder; let water flow slowly down vial wall; roll gently 30-60 seconds until dissolved (do not shake); inspect for clarity (GHK-Cu may show slight blue/green tint from copper; BPC-157 and TB-500 should be clear); label with reconstitution date and store at 2-8 °C (35.6-46.4 °F). TB-500 is the limiting reconstitution-stability window, most workflows use the reconstituted blend within 1-2 weeks.
peptidedosingprotocols.com
What it saysHide(secondary reference; provider confirmation required). [Source: peptidedosingprotocols.com]
10 units (0.10 mL) daily, approximately 2.33 mg total blend.not statednot statedAdd 3.0 mL bacteriostatic water to reconstitute.
Peptide Dosing Protocols stack
What it saysHide(secondary reference; provider confirmation required).
Glutathione
Immune Support · Inflammation · Longevity · Metabolic Health · Recovery
Standard: 250-1,000 mg/day · Low: 250 mg/day · High: 1,000 mg/dayOraldaily4 weeks - 6 months · 4 weeks or longer to appear and faded after stopping.reconstitution and supplies for injectable use, side effects, and where the evidence stops.
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Standard oral dosing ranges from 250 to 1,000 mg per day.
200-600 mg per injection (IM/SC); 600-1400 mg per session (IV push); 500-1000 mg daily (oral)subcutaneous or intranasal or oral or topical or intramuscular1-3 times per week (injectable); daily (oral)Key limitations / notesnot stated
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
HCG
Recovery
250-500 IU per injection (TRT adjunct); 1500-5000 IU (fertility protocols)subcutaneous or oral or topical or intramuscularEvery other day or 2-3 times per weekBacteriostatic water
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Humanin
Recovery
Standard: 4 mg/kg · Low: 2.5 mg/kg/injectionIP2x/week4-12 weeks · 6-8 weeksreconstituted with 2 mL bacteriostatic water (10 mg/mL)
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). The standard dosing for Humanin is 4 mg/kg administered via intraperitoneal injection twice weekly.
IGF-1 LR3
Recovery
Standard: 40 mcg/day · Low: 20 mcg/day · High: 100 mcg/daySubcutaneous (SubQ) or intramuscular (IM) injectionOnce daily4 weeks · 4 weeks on, 4 weeks offreconstituted in 2 mL of bacteriostatic water (500 mcg/mL)
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: 20-30 hours. Standard dosing is 40 mcg/day.
Ipamorelin
Sleep · Body Composition · Recovery · Performance Recovery · Longevity
Standard: 200-300 mcg/day · Low: 100 mcg/day · High: 300 mcgsubcutaneous1-3 times per day8-12 weeks on, then 4 weeks off · 8-12 weeks3.0 mL BAC water for 10 mg vial
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: about two hours. Most research protocols use 100 to 300 mcg per shot, one to three times per day.
200-300 mcg per injectionsubcutaneousnot stated8-12 weeks, then 2-4 weeks off · RepeatableBacteriostatic water
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Referenced at 100-300 mcg (0.1-0.3 mg) per dose. Commonly paired with CJC-1295 for synergistic GH release with minimal cortisol or prolactin elevation. Sample stacks cite 100 mcg each, fasted morning and before bed.subcutaneous2-3x daily (vitality contexts cite once daily)Morning fasted, pre-workout, or before bed · On-cycle 8-12 weeks, off-cycle 4 weeksnot stated
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
Kisspeptin-10
Recovery
Standard: 100-200 mcg · Low: 50-100 mcgSubcutaneous (SC)Once daily SC8 weeks: 56 doses needed; one vial still leaves margin for losses and schedule adjustments Insulin Syringes (U-100, 0.Reconstitute with bacteriostatic water (BAC water) before drawing any dose.
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: about 4 minutes. The standard dosing for Kisspeptin-10 is 100-200 mcg once daily.
9.6 nmol/kg SC (IVF trigger); 1 nmol/kg IV (diagnostic challenge)subcutaneousSingle dose (IVF trigger); single dose (diagnostic)Single administration per IVF · RepeatableSterile water
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
KLOW
Tissue Repair · Skin & tissue · Gut Health · Inflammation · Recovery
~6.0 mg total blend per day (22.5 units / 0.225 mL at 3.0 mL reconstitution) at maintenance phase; titration starts at ~2.0 mg total blend (7.5 units) and steps up to ~4.0 mg (15 units) then ~6.0 mg (22.5 units)subcutaneousOnce dailyStandard: 4-6 weeks on, 2-4 weeks off; Extended: 8-12 weeks on, 4 weeks off; Maximum extension: up to 16 weeks on, 4+ weeks off (no safety data supports continuous use beyond 12 weeks)Add 3.0 mL bacteriostatic water to the 80 mg vial to yield ~26.7 mg/mL total blend concentration. Steps: (1) Inspect the vial, check label, batch, and powder appearance; a discolored or damp vial should not be used. (2) Sanitize, swab the rubber stopper of the KLOW vial and the bacteriostatic water vial with alcohol prep pads and let dry. (3) Draw 3.0 mL bacteriostatic water with a sterile syringe; vent the BAC water vial if needed. (4) Inject down the vial wall, insert the needle and inject BAC water slowly down the inside wall; avoid spraying directly onto the powder to prevent foaming and denaturation. (5) Swirl, do not shake, gently swirl or roll until powder is fully dissolved; solution should be clear with possible light blue tint from GHK-Cu (normal). (6) Label with reconstitution date and store at 35.6-46.4°F (2-8°C), protected from light. (7) Use within 14-28 days when refrigerated; avoid freeze-thaw cycles.
peptidedosingprotocols.com
What it saysHide(secondary reference; provider confirmation required). [Source: peptidedosingprotocols.com]
Commonly, a once-daily subcutaneous injection is used, with a typical cycle of 4-6 weeks on and 2-4 weeks off.not statednot statedAdd 3.0 mL of bacteriostatic water to the 80 mg vial.
Peptide Dosing Protocols stack
What it saysHide(secondary reference; provider confirmation required).
KPV
Inflammation · Gut Health · Tissue Repair · Recovery
Standard: 200-500 mcg once or twice daily · Low: 200 mcg/day SubQ · High: 500 mcg twice dailyoral, subcutaneousonce or twice dailyWeeks 1-8+ · 4-8 weeks on, 2-4 weeks off2 mL BAC water
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). KPV is commonly dosed at 200-500 mcg once or twice daily for gut health.
200-500 mcg SC daily, or 250-500 mcg orally twice daily for gut-directed supportsubcutaneous or oralonce daily (SC) or twice daily (oral)4-8 weeks · 4-8 week cyclesCommonly 5 mg vials (injectable) or oral capsulesReconstitute with bacteriostatic water (injectable form)
Commonly published practitioner dosing range - provider confirmation required.
200-500 mcg per injection (SC); or oral capsules as directedsubcutaneous or oralOnce daily (SC); 1-2 times daily (oral)Bacteriostatic water
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
LL-37
Immune Support · Inflammation · Gut Health · Tissue Repair
100 mcg SC daily or every other daysubcutaneousdaily or every other day2-4 weeks · Short cycles; reassessCommonly 5 mg vialsReconstitute with bacteriostatic water
Commonly published practitioner dosing range - provider confirmation required.
100-200 mcg daily (SC); topical per formulationsubcutaneous or oral or topicalOnce daily, 5 days per week (SC); as directed (topical)2-4 weeks, then 2 weeks off · RepeatableBacteriostatic water
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Mazdutide
Metabolic Health · Body Composition · Weight Management · Appetite Regulation
2.5-5 mgsubcutaneousOnce weeklyTitrate over weeks per tolerance10 mg vial10 mg vial + 3.0 mL bacteriostatic water (~3.33 mg/mL). Draw 3.0 mL BAC water, inject slowly down the vial wall, swirl gently (do not shake), refrigerate at 2-8 C protected from light.
Peptide Dosages -
What it saysHide(secondary reference; provider confirmation required). Educational protocol, mirrors clinical-trial gradual titration. [Source: Peptide Dosages]
Melanotan I (MT-1)
Recovery
0.16 mg/kg daily (injection protocol); 16 mg implant every 2 months (approved)subcutaneous or oralDaily for 10 days per10-day injectionSterile water
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Melanotan II (MT-2)
Recovery
Loading: 0.25-0.5 mg daily; Maintenance: 0.5-1.0 mg 1-2 times per weeksubcutaneous or oralDaily during loading (1-3 weeks); 1-2 times per week for maintenanceLoading: 2-4 weeks; Maintenance: ongoing as desiredBacteriostatic water
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Standard: 500-1000 mcg daily · Low: 100-250 mcg daily · High: 250-500 mcg dailySubcutaneous (SubQ) injection1-2x per week4-6 weeks · 6 weeks2 mL BAC water
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: half-life Melanotan 2 ~1-2 hours Afamelanotide(MT-I) ~0. The standard loading dose is 500-1000 mcg daily.
Methylene Blue
Recovery
Standard: 1 mg/kg · Low: 5-20 mg/day · High: 10 mg/kgIV, Oralnot statedno
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Methylene blue is typically dosed at 1 mg/kg for methemoglobinemia.
MOTS-c
Metabolic Health · Longevity · Performance Recovery · Recovery
Standard: 5 mg · Low: 5 mg · High: 10 mgSubcutaneous injection2 to 3 times per week4 to 8 weeks · 4-6 weeksReconstitute a 10 mg vial with 1.0 mL bacteriostatic water
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). The standard dosing is 5 mg subcutaneously, 3 times per week for 4-6 weeks.
5-10 mg SC 2-3 times weeklysubcutaneous2-3 times weekly4 weeks · 4-week cyclesCommonly 10 mg vialsReconstitute with bacteriostatic water
Commonly published practitioner dosing range - provider confirmation required.
5-10 mg per injectionsubcutaneous2-3 times per week or every 5 daysBacteriostatic water
Peptide Protocol Wiki
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Referenced at 10 mg weekly in divided doses within longevity stacking. Morning administration cited for circadian and mitochondrial alignment.subcutaneousWeekly total (10 mg), divided dosesMorning · Cycle every few months in longevity contextsnot stated
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
NAD+
Longevity · Metabolic Health · Cognitive Support · Recovery
Standard: 100 mg · Low: 50 mg · High: 200 mgSubQ2-3x weekly4-8 weeks · 4 weeks, then escalate to 100 mg weekly.7.5 mL BAC water for 750 mg vial
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). The standard dose for NAD+ is 100 mg administered 2-3 times weekly.
250-750 mg per IV session; 300-1000 mg daily for oral precursors (NR/NMN)oral1-2x weekly IV (loading), monthly (maintenance); daily oral4-8 week loading phase IV; ongoing for oral precursorsNormal saline (0.9% NaCl) 250-500 mL for IV
Peptide Protocol Wiki
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Oxytocin Acetate
Recovery
Standard: 200-300 mcg · Low: 100 mcg · High: 400-500 mcgSubcutaneousOnce dailycycle Duration 1-2 weeks Review Point Week 2 Notes Used in mood, anxiety, and pain research blocks.add 2-3 mL bacteriostatic water to a 2 mg or 5 mg lyophilized vial
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: about 3-6 minutes. The standard dosing for subcutaneous oxytocin is 200-300 mcg once daily.
24 IU intranasal (6 sprays of 4 IU each, 3 per nostril)intranasalOnce or twice daily for research protocolsnonapeptide neurohormone produced in the hnot stated
Peptide Protocol Wiki
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P21
Recovery
Practitioner ranges vary; commonly ~1 mg SC daily - provider-directedsubcutaneousonce dailyPer provider directionCompounded; variesReconstitute with bacteriostatic water
Commonly published practitioner dosing range - provider confirmation required.
Pinealon
Recovery
Standard: 1.0 to 2.0 mg per day · Low: 1.0 mg · High: 2.0 mgsubcutaneous injection or oralOnce daily10 to 20 days · 2 to 3 cycles per year3.0 mL BAC water
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Pinealon is typically dosed at 1.0 to 2.0 mg per day.
PT-141
Recovery
Standard: 1.75 mgSubcutaneous (abdomen or thigh)1 dose per 24 hours8 doses per monthreconstitution math, route considerations, and side effects for this bremelanotide peptide.
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). The standard dose of PT-141 is 1.75 mg subcutaneous at least 45 minutes before sexual activity.
1.75 mgsubcutaneous or oralnot statednot stated
Peptide Protocol Wiki
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Injection referenced at 0.5-2 mg per dose with onset in 1-4 hours, peak at 6-12 hours, duration 24-72 hours. Nasal spray referenced at 2-4 sprays per nostril, onset 30-60 minutes, duration 6-12 hours. Start low; side effects are dose-dependent (nausea most common).subcutaneous or nasalAs neededOn-demand, 1-4 hours before desired effect (injection)not stated
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
Retatrutide
Metabolic Health · Body Composition · Weight Management · Appetite Regulation
Standard: 1 mg titrating to 12 mg once weekly (Phase 3 TRIUMPH: 1 mg → 2 mg → 4 mg → 6 mg → 9 mg → 12 mg, stepping up every 4 weeks) · Low: 1 mg once weekly · High: 12 mg once weeklySubcutaneous injectionOnce per week, on the same day each week48 weeks (Phase 2 reference); 68-72 weeks (Phase 3 TRIUMPH reference) · Every 4 weeks at each dose step; review at 4 mg, 9 mg, 12 mg; slow titration option adds 2-4 weeks per dose step when GI symptoms persist10 mg vial with 2.0 mL BAC water yields a concentration of 5 mg/mL; use U-100 insulin syringes
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Retatrutide is dosed once weekly via subcutaneous injection starting at 1 mg and titrating up every 4 weeks to a maximum of 12 mg following the Phase 3 TRIUMPH schedule.
1-12 mgsubcutaneousOnce weeklyAny time of day · Titrate over weeks per response/tolerancenot stated
Reference titration range, provider confirm
Retatrutide / MOTS-c
Metabolic Health · Body Composition · Weight Management · Mitochondrial Function
Retatrutide: titrate 1 → 2 → 4 → 6 → 9 mg weekly (maintenance 9 mg or 12 mg weekly); MOTS-c intermittent: 5 mg 2-3x weekly (maintenance 5-10 mg 2-3x weekly); MOTS-c low-dose daily alternative: start 200 mcg/day up to 1 mg/daysubcutaneousRetatrutide: once weekly; MOTS-c: 2-3x weekly (intermittent) or daily (low-dose alternative)Conservative starter: retatrutide weeks 1-24, MOTS-c weeks 5-12 then off; Standard research cycle: retatrutide weeks 1-48, MOTS-c 8-week pulses with 4-week breaks; Aggressive deficit: retatrutide weeks 1-68, MOTS-c 6 weeks on / 2 weeks off repeatingStandard 7-Step Reconstitution: (1) Let the lyophilized vial and BAC water reach room temperature before opening. (2) Swab both vial stoppers with alcohol and let them dry. (3) Pull the planned BAC water volume into a sterile syringe. (4) Inject the BAC water slowly down the inside of the vial wall, do not aim at the powder. (5) Swirl or roll gently until fully dissolved; do not shake. (6) Confirm the solution is clear; label the vial with compound name, concentration, and date. (7) Store at 2-8 °C; use retatrutide within 28-60 days and MOTS-c within 7 days for best potency. Each compound is reconstituted separately. Retatrutide common concentration: 10 mg vial + 1.0 mL BAC water = 10 mg/mL. MOTS-c common concentration: 10 mg vial + 2.0 mL BAC water = 5 mg/mL. MOTS-c aliquots may be stored at -20 °C for 2-3 months.
peptidedosingprotocols.com
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RT3
Recovery
Standard: 1 mg once weekly · Low: 1 mg · High: 12 mgSubcutaneous injectiononce per weeknullnull
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: null. The standard dosing starts at 1 mg once weekly and titrates up to 12 mg.
0.5 mg starting dose, escalating to target of 8-12 mgsubcutaneous or oralOnce weeklyKey Questionnot stated
Peptide Protocol Wiki
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Russian Nootropic Stack
Cognitive Function · Longevity · Neuroprotection
Semax: 300-800 mcg/day (SubQ), 400-600 mcg/day (intranasal); Selank: 250-500 mcg/day (SubQ), 400-750 mcg/day (intranasal). Maintenance example: Semax 600 mcg + Selank 400 mcg daily.Subcutaneous injection or intranasal (drops/spray)Once daily (SubQ, morning before 2 PM); 2x daily for Semax intranasal (AM + early afternoon); 2-3x daily for Selank intranasalStandard cycle: 4 weeks on / 4 weeks off; Short intensive: 10-14 days on / 10-14 days off; Extended: 8 weeks (5 days/week) / 4 weeks off; Clinical intranasal reference: 14-21 days on / 14-21 days offEach compound is reconstituted separately using bacteriostatic water (BAC water). For a 10 mg vial: add 3.0 mL BAC water to reach 3,333 mcg/mL, or 2.0 mL to reach 5,000 mcg/mL. For a 5 mg vial: add 2.0 mL BAC water to reach 2,500 mcg/mL, or 1.0 mL to reach 5,000 mcg/mL. Use 0.3 mL / 30-unit insulin syringes for accuracy at small volumes. Label each vial with compound name, concentration, and reconstitution date. Semax is light-sensitive (contains methionine, which oxidizes when exposed to light); wrap the reconstituted vial in aluminum foil or store in a dark container. Both vials are stable for approximately 30 days at 2-8 °C once reconstituted.
peptidedosingprotocols.com
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Selank
Neuroinflammation · Sleep
Standard: 250-500 mcg per day · Low: 250 mcg · High: 500 mcgSubcutaneous injection (SubQ) or intranasal nasal sprayonce daily for SubQ; 14-21 days for intranasal4 weeks for SubQ; 14-21 days for intranasal · 4 weeks on, 4 weeks off for SubQ; 1-3 weeks off for intranasal3 mL, 6 mL, or 10 mL per 30 mg vial
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: Half-life Selank ~2-10 min plasma; 12-24 hr functional duration Semax ~3-5 min plasma; 20-24. The standard research-use SubQ schedule is 4 weeks on at 250-500 mcg per day.
250-500 mcg intranasally daily (or 0.15% solution per protocol)intranasalonce or twice daily10-14 day cycles · Repeat cycles per provider directionCompounded intranasal solution/sprayCompounded intranasal solution - no reconstitution required
Commonly published practitioner dosing range - provider confirmation required.
300-600 mcg per dose intranasal; 200-400 mcg per dose subcutaneoussubcutaneous or intranasal or oral2-3 times daily (intranasal); once daily (SC, 5 days per week)30 days on, 30 days off; approximately 6not stated
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Referenced at 250-500 mcg (0.25-0.5 mg) per dose. Nasal spray cited as the most common route; onset within 30-60 minutes, peak benefit after several days of consistent use.nasal or subcutaneousNasal 1-3x daily; injection once daily or as needednot stated
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
Semaglutide
Metabolic Health · Body Composition · Weight Management · Appetite Regulation
Standard: 2.4 mg · Low: 0.25 mg · High: 2.4 mgsubcutaneous injectiononce weekly16 weeks · 16 weeks on, ongoing weekly maintenancereconstituted lyophilized vial
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: about 7 days. Standard dosing escalates to 2.4 mg over 16 weeks.
Semax
Neuroinflammation
Standard: 0.6 to 1.5 mg/day · Low: sub-milligram per administration · High: up to ~1 mg/kgIntranasal solution, Subcutaneous injectionDaily administration, often divided into morning and afternoon doses10-14 days for standard short course, 4-6 weeks for extended cognitive course · 4-6 weeks on, 2-4 weeks offRequires reconstitution with bacteriostatic water before subcutaneous research use
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Standard dosing for Semax is about 0.6 to 1.5 mg per day, typically split into two administrations.
300-600 mcg intranasally daily (or 1-3 drops/nostril of 0.1% solution)intranasalonce or twice daily10-14 day cycles · Repeat cycles per provider directionCompounded intranasal solution/sprayCompounded intranasal solution - no reconstitution required
Commonly published practitioner dosing range - provider confirmation required.
200-600 mcg per dose (0.1% solution) for nootropic use; 3-6 mg per dose (1% solution) for strokeintranasal2-3 times dailyRepeatablenot stated
Peptide Protocol Wiki
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Referenced at 300-600 mcg (0.3-0.6 mg) daily in brain health protocols. Morning administration cited to carry cognitive benefit through the day. Rotation with other nootropic peptides between cycles referenced to maintain response.nasalDailyMorning · 4 weeks on with rotation between cyclesnot stated
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
Sermorelin Acetate
Longevity · Sleep · Body Composition · Metabolic Health · Recovery
Standard: 200-300 mcg · Low: 100 mcg · High: 400-500 mcgsubcutaneous injectionnightly3-6 months · Cycle length Planning note 4 weeks 1 vial 28 nights of 200 mcg from one vial.3 mL bacteriostatic (BAC) water in a 10 mg vial gives 3 units per 100 mcg on a U-100 insulin syringe.
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: 11-12 minutes. Common dose is 200-300 mcg subQ nightly.
200-300 mcgsubcutaneous or oralOnce daily at bedtime, 5 nights per week3-6 monthsBacteriostatic water
Peptide Protocol Wiki
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SLU-PP-322
Recovery
Standard: 50 mg (1 tablet) · Low: ≤1.5 mg · High: 100 mg (2 tablets)oralonce daily6 weeks IP dosing (Burris-lab standard) Improved ejection fraction; reduced fibrosis; increased survival in the TAC model.5 mg vial reconstituted with 2 mL BAC water (2,500 mcg/mL) and a daily 500 mcg subcutaneous draw, a common community pattern, not a clinically validated dose.
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: half-life, there is no validated missed-dose rule. The standard dosing for SLU-PP-332 is 50 mg taken once daily.
SNAP-8
Recovery
3-10% concentration in formulation (solution, not pure peptide)topicalTwice daily (morning and evening)Continuous use; minimum 28 days for measurable resultsnot stated
Peptide Protocol Wiki
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SS-31
Mitochondria
40 mg subcutaneous daily (Phase 3 dose); or 5-10 mg daily (research community range)subcutaneousOnce dailyRepeatablenot stated
Peptide Protocol Wiki
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Standard: 40 mg · Low: 20 mgSubcutaneousonce daily12 weeks per period Open-label extension All remaining patients on elamipretide 40 mg SC daily Up to 168 weeks Reported in Genetics in Medicine, 2024.reconstitution, and safety considerations for this mitochondria-targeted peptide.
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). The standard dosing for SS-31 is 40 mg subcutaneously once daily.
Investigational; commonly studied at 40 mg SC daily - provider-directedsubcutaneousonce daily (study protocols)Per provider/study protocolCompounded; variesReconstitute per compounding pharmacy instructions
Commonly published practitioner dosing range - provider confirmation required.
Survodutide
Recovery
Standard: 4.8 mg once weekly · Low: 0.6 mg once weekly · High: 4.8 mg once weeklySubcutaneous injectiononce weekly76 weeks · null1.0 to 2.0 mL per vial
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: null. Survodutide is administered at a standard dose of 4.8 mg once weekly.
TA1
Immune Support · Inflammation · Recovery · Post-Procedure Recovery
Standard: 1.6 mgSubcutaneousTwice weekly6 to 12 months · 12 weeks Community-derived, not from a clinical trial These durations are reported from research, not personal-use recommendations.2 mL bacteriostatic water
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: half-life of about 2-3 hours. The standard dosing for TA1 is 1.6 mg subcutaneously twice weekly.
1.5 mg SC twice weekly, or 450-900 mcg dailysubcutaneoustwice weekly, or daily4-12 weeks · Repeat course per provider directionCommonly 5 mg or 10 mg vialsReconstitute with sterile/bacteriostatic water
Commonly published practitioner dosing range - provider confirmation required.
1.6 mgsubcutaneous or oralTwice weekly (standard); daily during chemotherapySterile water
Peptide Protocol Wiki
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Cycling reference for immune support: 6-12 weeks on, 6 weeks off.subcutaneousnot statedOn-cycle 6-12 weeks, off-cycle 6 weeksnot stated
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
TB-500 / TB4
Tissue Repair · Soft Tissue Recovery · Tendon/Ligament Recovery · Injury Recovery · Performance Recovery · Recovery
Standard: 2-2.5 mg subcutaneous twice weekly · Low: 2 mg twice weekly · High: 2.5 mg subcutaneous once weeklysubcutaneous injectiontwice weekly for loading, once weekly for maintenanceWeeks 1-6 for loading, Weeks 7-12 for maintenance · 4-12 weeks2.0 mL bacteriostatic water for 10 mg vial
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). TB-500 is commonly dosed at 2-2.5 mg subcutaneously twice weekly during the loading phase.
2-2.5 mg SC twice weekly (loading 4-6 weeks), then 2 mg weekly maintenancesubcutaneoustwice weekly (loading), then weekly4-6 week loading, then maintenance · Loading phase then maintenance phaseCommonly 5 mg or 10 mg vialsReconstitute with bacteriostatic water
Commonly published practitioner dosing range - provider confirmation required.
2-2.5 mg per injection (loading); 750 mcg-2 mg (maintenance)subcutaneous or oral or topicalnot statedBacteriostatic water
Peptide Protocol Wiki
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Referenced with a loading phase of 5-10 mg weekly for 4-6 weeks, then maintenance at 2-5 mg weekly split into smaller doses. Results typically noticed within 2-4 weeks. Caution flag: angiogenesis-promoting, so cancer or tumor history warrants medical consultation first.subcutaneous or intramuscularWeekly total (2-10 mg) split into smaller dosesPost-workout or before bed · On-cycle 4-6 weeks, off-cycle 1-2 monthsnot stated
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
Tesamorelin
Metabolic Health · Body Composition · Cognitive Support
Standard: 2 mg once a daySubcutaneous injection in the abdomen onlyOnce daily26 weeks · Cycle length Planning note 4 weeks 6 vials (10 mg) 28 daily doses needed.Reconstitute with bacteriostatic water
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: Half-Life Tesamorelin 26-38 minutes Sermorelin 11-12 minutes CJC-1295(DAC) ~6-8 days Fe. The standard dosing for Tesamorelin is 2 mg administered once daily.
1-2 mg SC daily at bedtimesubcutaneousonce daily at bedtime12+ weeks · Continuous per provider directionCommonly 2 mg vialsReconstitute with sterile/bacteriostatic water
Commonly published practitioner dosing range - provider confirmation required.
2 mgsubcutaneousOnce dailynot stated
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Fat loss stacking reference: 200 mcg (0.2 mg) fasted morning paired with AOD-9604 300 mcg. Cited for visceral fat targeting with Mod GRF 1-29 as a pulse-enhancing partner.subcutaneousDaily in stack contextsMorning, fastednot stated
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
Tesamorelin / Ipamorelin
Body Composition · Metabolic Health · Recovery · Longevity
Tesamorelin 1-2 mg + Ipamorelin 200-300 mcg (separate vials); or blend draws of 10-40 units/day depending on vial ratio (5/5 vial example: up to 667 mcg tesamorelin + 667 mcg ipamorelin at 40 units)subcutaneousOnce daily subcutaneousStandard: 8-12 weeks on, 4-8 weeks off; Extended: 12-16 weeks on, 4-8 weeks off; typical cycle 8-16 weeksAdd 3.0 mL bacteriostatic water to the 3 mL vial, aiming the stream down the inside vial wall; swirl gently (do not shake) until fully dissolved. Inspect: solution should be clear; discard if cloudy or full of particles. Label with ratio, date, and concentration. Refrigerate at 2-8°C (35.6-46.4°F), protected from light. 10/3 vial yields ~4.33 mg/mL total (3.33 mg/mL tesamorelin + 1.0 mg/mL ipamorelin); 5/5 vial yields ~3.33 mg/mL total (1.67 mg/mL each). Do not freeze reconstituted solution.
peptidedosingprotocols.com
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Tesofensine
Recovery
Standard: 0.5 mg once daily · Low: 0.25 mg once dailyOral tabletonce daily24 weeks · 24 weeks onnot stated
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: about 9 days. The standard dosing for tesofensine is 0.5 mg taken once daily.
Tirzepatide
Metabolic Health · Body Composition · Weight Management · Appetite Regulation
2.5-15 mgsubcutaneousOnce weeklyAny time of day · Titrate over weeks per response/tolerancenot stated
Reference titration range, provider confirm
Standard: 5 mg, 10 mg, or 15 mg once weekly (three approved maintenance doses) · Low: 2.5 mg once weekly · High: 15 mg once weeklySubcutaneous injectionOnce weeklyWeeks 1-4 at 2.5 mg, stepping up every 4 weeks; full ladder to 15 mg reached at week 21+ · 4 weeks (Phase 1, 2.5 mg), 8 weeks (through 5 mg), 12 weeks (through 7.5 mg), 16 weeks (through 10 mg), 24 weeks (full ladder to 15 mg)40 mg vial + 2.0 mL BAC water = 20 mg/mL; reconstituted solution keeps for ~28 days refrigerated
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: 5 days. Tirzepatide is titrated once weekly from 2.5 mg up to a maximum of 15 mg in 2.5 mg increments every 4 weeks via subcutaneous injection.
TRZ
Recovery
Standard: 5 mg once weekly · Low: 2.5 mg once weekly · High: 15 mg once weeklySubcutaneous injectiononce weeklyCycle length Planning note 4 weeks (Phase 1, 2.2.0 mL BAC water for a 40 mg vial
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: 5 days. Tirzepatide is administered once weekly, starting at 5 mg.
2.5 mg starting dose, escalating to 5-15 mg maintenancesubcutaneous or oralOnce weeklyOngoing (chronic therapy)not stated
Peptide Protocol Wiki
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VIP
Immune · Neuroinflammation
50 mcg per spray, 4-8 sprays daily (CIRS/nasal); 50-150 pmol/kg/min (IV infusion)intranasal or oralnot statedSterile 0.9% saline
Peptide Protocol Wiki
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Standard: 100 mcg · Low: 50 mcg · High: 200 mcgSubQ1-2x dailyOngoing · 12 week cycles.A 10 mg subcutaneous vial with 3 mL bacteriostatic water gives about 3.33 mg/mL.
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: very short. The standard dosing for VIP is 100 mcg taken 1-2 times daily.
50 mcg per spray intranasally, up to 4 times daily (titrate per CIRS protocol; typically after correcting other abnormalities)intranasalup to 4 times dailyPer provider directionCompounded intranasal spray (e.g. 50 mcg/actuation)Compounded intranasal spray - no reconstitution required
Commonly published practitioner dosing range - provider confirmation required.
Vladonix
Recovery
1-2 capsules daily (commonly 2 capsules daily)oralonce or twice daily20-30 day course · Repeat course 1-2x per yearOral capsules (peptide bioregulator)Not applicable (oral capsule)
Commonly published practitioner dosing range - provider confirmation required.
Wolverine
Tissue Repair · Soft Tissue Recovery · Injury Recovery · Recovery
Pre-blended: 0.5-2.0 mg total blend daily (0.25-1.0 mg BPC-157 + 0.25-1.0 mg TB-500); Separate vials: BPC-157 250-500 mcg daily, TB-500 2.0-5.0 mg 2x/week (loading), TB-500 2.0 mg 1-2x/week (maintenance)subcutaneousPre-blended: daily; Separate vials: BPC-157 daily, TB-500 2x/week (loading) or 1-2x/week (maintenance)6-12 weeks total; loading phase weeks 1-4, maintenance phase weeks 5-8 or 5-12 depending on protocol; off-cycle gaps of 2-4 weeks commonly describedPre-blended 20 mg vial: Add 2.0 mL bacteriostatic water → 10 mg/mL total (5 mg/mL each compound); 10 units (0.10 mL) delivers 0.5 mg BPC-157 + 0.5 mg TB-500; ~20 doses per vial at 1.0 mg/day. Separate vials: Add 2.0 mL BAC water to each 10 mg vial → 5.0 mg/mL each. Steps: allow lyophilized peptide to reach room temperature; wipe both vial stoppers with alcohol swabs; draw planned BAC water volume into sterile syringe; inject BAC water against inside wall of peptide vial slowly, never directly onto powder; swirl gently until fully dissolved, do not shake; verify solution is clear (TB-500 may appear very faintly tinted); refrigerate at 35.6-46.4°F (2-8°C) and use within supplier's stated beyond-use window.
peptidedosingprotocols.com
What it saysHide(secondary reference; provider confirmation required). [Source: peptidedosingprotocols.com]
1.0 mg total blend daily (0.5 mg of each compound) from a pre-blended 20 mg vial.not statednot statedReconstitute with 2.0 mL BAC water for a final concentration of 10 mg/mL total blend (5 mg/mL each component).
Peptide Dosing Protocols stack
What it saysHide(secondary reference; provider confirmation required).
5-Amino-1MQ
Recovery
Standard: 100 mg once daily · Low: 50 mg once daily · High: 150 mg once dailyoral
Frequency
once daily
Duration
8 to 12 weeks on, 4 to 6 weeks off
Reconstitution
Reconstitute each vial with 3.0 mL bacteriostatic (BAC) water for ~16.7 mg/mL; 2 vials = ~6 mL total. (Subcutaneous form; the compound is also taken orally.)
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: 4 to 7 hours. The most common research community target dose is 100 mg once daily.
Advanced Recomp Stack
Body Composition · Metabolic Health · Recovery · Performance
Retatrutide: 2 mg/week (initiation, weeks 1-4) escalating to 12 mg/week (maximum, weeks 17-20+); CJC-1295 (No DAC): 100-200 mcg, 1-2x daily; Ipamorelin: 100-200 mcg, 1-2x dailysubcutaneous
Frequency
Retatrutide: once weekly; CJC-1295 and ipamorelin: 1-2x daily (5-on / 2-off rhythm)
Duration
Standard cycle 12-16 weeks; Extended cycle 16-24 weeks; Continuous retatrutide + cycled CJC/Ipa (12 weeks on / 4 weeks off). CJC/Ipa cycled 12 weeks on / 4 weeks off; retatrutide can continue.
Reconstitution
Retatrutide: 5 mg vial + 1 mL BAC water = 5 mg/mL; 10 mg vial + 2 mL BAC water = 5 mg/mL; 10 mg vial + 1 mL BAC water = 10 mg/mL; 20 mg vial + 2 mL BAC water = 10 mg/mL. Volumes over 1.0 mL need a larger syringe or a split injection at two sites. CJC-1295 (No DAC): 2 mg vial + 1 mL BAC water = 2,000 mcg/mL; 5 mg vial + 2 mL BAC water = 2,500 mcg/mL; 5 mg vial + 2.5 mL BAC water = 2,000 mcg/mL. Ipamorelin: same math as CJC-1295. Pre-blended CJC/Ipa combo vial (10 mg = 5 mg CJC + 5 mg ipamorelin): reconstitute with 2 mL BAC water for 2,500 mcg/mL per peptide; 8 units (0.08 mL) = ~200 mcg of each. Label every vial with compound name, concentration, and reconstitution date. Retatrutide is dosed in mg; CJC/Ipa in mcg, a 1,000x difference. Use U-100 insulin syringes.
Reported by
peptidedosingprotocols.com
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AOD-9604
Metabolic Health · Body Composition · Recovery
Standard: 300-500 mcg/day · Low: 300 mcg · High: 500 mcgSubcutaneous injection
Frequency
daily
Duration
12-24 weeks
Cycle
Cycle context Phase 2 protocols ran 12-24 weeks.
Reconstitution
3.0 mL bacteriostatic water for a 5 mg vial
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Common research dose is 300-500 mcg/day.
300 mcg SC daily (commonly AM, fasted)subcutaneous
Frequency
once daily
Duration
8-12 weeks
Cycle
8-12 week cycles
Vial
Commonly 2 mg or 5 mg vials
Reconstitution
Reconstitute with bacteriostatic water
Reported by
Commonly published practitioner dosing range - provider confirmation required.
250-300 mcgsubcutaneous or oral
Frequency
Once daily
Duration
8-12 weeks
Reconstitution
Bacteriostatic water
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Referenced at 250-500 mcg once daily, up to 500 mcg-1 mg for aggressive fat loss protocols. Fasted morning administration cited. Noticeable results reported within 4-6 weeks.subcutaneous
Frequency
Once daily
Timing
Morning, fasted, before eating
Cycle
12 weeks on followed by a short break to prevent desensitization
Reported by
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
ARA-290
Recovery
4 mgsubcutaneous or intranasal or oral
Frequency
Once daily
Duration
28 days
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
BPC-157
Tissue Repair · Soft Tissue Recovery · Tendon/Ligament Recovery · Gut Health · Injury Recovery · Recovery
Standard: 250 mcg twice daily · Low: 250 mcg once daily · High: 500 mcg twice dailySubcutaneous injection
Frequency
Twice daily
Duration
4-6 weeks
Cycle
Standard
Reconstitution
2 mL BAC water
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: under 30 minutes. The standard dosing for BPC-157 is 250 mcg twice daily.
250-500 mcgsubcutaneous or oral or topical
Frequency
Twice daily (morning and evening)
Duration
4-6 weeks
Reconstitution
Bacteriostatic water
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
250-500 mcg once or twice dailysubcutaneous
Frequency
once or twice daily
Duration
4-8 weeks
Cycle
4-8 week cycles, may repeat after a break
Vial
Commonly supplied as 5 mg or 10 mg vials
Reconstitution
Reconstitute lyophilized powder with bacteriostatic water; refrigerate after mixing
Reported by
Commonly published practitioner dosing range - provider confirmation required.
Referenced at 200-500 mcg (0.2-0.5 mg) daily overall; recovery contexts cite 200-400 mcg once or twice daily, commonly right after training. Oral form referenced as viable for gut-focused use.subcutaneous, intramuscular near injury, or oral
Frequency
1-2x daily
Timing
Morning for systemic or gut focus; post-workout for tissue repair; before bed for overnight healing
Cycle
On-cycle 4-6 weeks, off-cycle 1-2 months
Reported by
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
Cagrilintide
Recovery
Standard: 2.4 mg/week · Low: 0.25 mg/week · High: 4.5 mg/weekSubcutaneous injection
Frequency
once weekly
Cycle
16 weeks
Reconstitution
BAC water added per vial; concentration depends on volume chosen.
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: About 159 to 195 hours(~7 to 8 days). Cagrilintide is dosed starting at 0.25 mg once weekly, escalating to 2.4 mg weekly.
Cagrilintide / Retatrutide
Metabolic Health · Body Composition · Weight Management · Appetite Regulation
Retatrutide 12 mg/week + Cagrilintide 2.4 mg/week (maintenance); pre-blend most common as 12.5 mg retatrutide + 2.5 mg cagrilintide per vialSubcutaneous
Frequency
Once weekly
Duration
24-week ramp + open-ended maintenance; community: 16-24 weeks on, 4-8 weeks off
Reconstitution
Pre-blend (15 mg vial: 12.5 mg retatrutide + 2.5 mg cagrilintide): add 2.5 mL bacteriostatic water (BAC water) slowly down the vial wall; resulting concentration is 5.0 mg/mL retatrutide + 1.0 mg/mL cagrilintide (6.0 mg/mL total blend). Both compounds use U-100 insulin syringes. BAC water is added slowly down the vial wall. Separate vials each use 1-2 mL BAC water per vial. Reconstituted vials are stable for 28-30 days at 2-8 °C.
Reported by
peptidedosingprotocols.com
What it saysHide(secondary reference; provider confirmation required). [Source: peptidedosingprotocols.com]
Cagrilintide / Tirzepatide
Metabolic Health · Body Composition · Weight Management · Appetite Regulation
Tirzepatide 15.0 mg/week + Cagrilintide 2.4 mg/week (maintenance); sub-maximal example: tirzepatide 10 mg + cagrilintide 1.2 mgSubcutaneous
Frequency
Once weekly
Duration
24-week ramp + open-ended maintenance; or stabilize tirzepatide at 10-15 mg then add cagrilintide for 12+ weeks; or sub-maximal combination as needed
Reconstitution
Each vial is reconstituted separately using bacteriostatic water (BAC water). Tirzepatide: 5 mg vial + 1.0 mL BAC = 5 mg/mL; 10 mg vial + 2.0 mL BAC = 5 mg/mL; 10 mg vial + 1.0 mL BAC = 10 mg/mL; 30 mg vial + 3.0 mL BAC = 10 mg/mL. Cagrilintide: 5 mg vial + 2.0 mL BAC = 2.5 mg/mL; 10 mg vial + 2.0 mL BAC = 5.0 mg/mL; 10 mg vial + 1.0 mL BAC = 10.0 mg/mL. Cagrilintide is sensitive, inject BAC water down the vial wall and swirl gently; do not shake (amyloid fibril risk); discard cloudy solution. Label every vial with compound name, concentration, and reconstitution date. Both compounds are stable 28-30 days at 2-8 °C once reconstituted. Standard steps: let vial and BAC water reach room temperature (~10-15 minutes); swab both vial stoppers with alcohol. Do not mix the two compounds in one syringe.
Reported by
peptidedosingprotocols.com
What it saysHide(secondary reference; provider confirmation required). [Source: peptidedosingprotocols.com]
CagriSema
Metabolic Health · Body Composition · Weight Management · Appetite Regulation
cagrilintide 2.4 mg + semaglutide 2.4 mgSubcutaneous
Frequency
Once weekly
Duration
REDEFINE trials ran 68 weeks; research cycles are commonly 12-24 weeks; short investigative cycles 8-12 weeks.
Reconstitution
1. Wash your hands and lay out a clean work surface. 2. Wipe the rubber stopper of each vial with an alcohol swab and let it dry. 3. Draw the planned BAC water volume into a fresh syringe. 4. Inject the BAC water down the inside wall of the peptide vial, do not spray directly onto the lyophilized powder. 5. Swirl gently until the powder is fully dissolved. Do not shake. 6. Label the vial with the date of reconstitution and the concentration. 7. Refrigerate at 36-46°F (2-8°C) and use within the supplier-specified stability window. For pre-blended vials: BAC water volume is dictated by the vial concentration printed on the COA, there is no universal default for a fixed-dose combination, so verify the supplier's spec before reconstituting. Illustrative examples: 20 mg vial + 2.0 mL BAC water = 10 mg/mL (0.48 mL = 48 units U-100 per weekly maintenance draw); 20 mg vial + 2.5 mL BAC water = 8 mg/mL (0.60 mL = 60 units U-100); 20 mg vial + 3.0 mL BAC water = 6.67 mg/mL (0.72 mL = 72 units U-100). For separate vials: reconstitute each compound independently; e.g. 5 mg vial + 2.0 mL BAC water = 2.5 mg/mL (0.96 mL = 96 units U-100 per 2.4 mg draw); 10 mg vial + 3.0 mL BAC water = 3.33 mg/mL (0.72 mL = 72 units U-100 per 2.4 mg draw).
Reported by
peptidedosingprotocols.com
What it saysHide(secondary reference; provider confirmation required). [Source: peptidedosingprotocols.com]
CJC-1295
Body Composition · Metabolic Health · Sleep · Recovery · Performance Recovery
100 mcg per injection (1-2 mcg/kg saturation dose)subcutaneous
Frequency
2-3 times daily
Duration
8-12 weeks
Reconstitution
Bacteriostatic water
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Standard: 200 mcg, once daily · Low: 100 mcg, once daily · High: 300 mcg, up to 3x dailySubcutaneous (SubQ)
Frequency
1-3 times daily
Duration
8-12 weeks
Cycle
4-6 weeks off
Reconstitution
10 mg vial with 3 mL bacteriostatic water
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: About 30 minutes. The standard dose is 200 mcg, administered once daily.
Non-DAC version referenced at 100-300 mcg (0.1-0.3 mg) per dose due to short half-life. Empty stomach preferred; avoid co-administration with carbohydrates or fats (insulin interference with GH release).subcutaneous
Frequency
1-3x daily
Timing
Morning, pre-workout, or before bed
Cycle
On-cycle 8-12 weeks, off-cycle 6-12 weeks
Reported by
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
CJC-1295 (DAC)
Recovery
Standard: 1,000 mcg once weekly · Low: 500-1,000 mcg once weekly · High: 2,000 mcg once weekly or 1 mg twice weeklySubcutaneous injection
Frequency
once weekly
Duration
8-12 weeks
Cycle
8-12 weeks on, 4-6 weeks off
Reconstitution
reconstitute lyophilized powder with bacteriostatic water before use
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: 6-8 days. Common research-planning ranges land between 1-2 mg subcutaneously once weekly.
DAC version referenced at 2-3 mg per dose; extended half-life supports infrequent dosing. Bedtime administration cited to align with sleep-phase GH release.subcutaneous
Frequency
1-2x weekly
Timing
Optionally before bed
Cycle
On-cycle 8-12 weeks, off-cycle 6-12 weeks
Reported by
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
CJC-1295 / Ipamorelin (GH Pulse)
Recovery · Body Composition · Longevity · Sleep
200-300 mcg of each peptide (CJC-1295 no DAC and Ipamorelin) daily; lower doses of 100-150 mcg typical for first 2-4 weeks before titrationsubcutaneous
Frequency
Once daily
Duration
Standard: 8-12 weeks on, 4 weeks off; Extended: 16 weeks on, 8 weeks off; Short test: 4-6 weeks on, 4 weeks off
Reconstitution
For a 20 mg pre-blended vial (10 mg CJC-1295 + 10 mg Ipamorelin): add 3.0 mL bacteriostatic water for a concentration of 6.67 mg/mL total (3.33 mg/mL each peptide); a 200 mcg dose of each peptide = 6 units (0.06 mL) on a U-100 syringe. Alternative fills: 2.0 mL BAC water = 10.0 mg/mL total (5.0 mg/mL each), 200 mcg dose = 4 units (0.04 mL); 2.5 mL BAC water = 8.0 mg/mL total (4.0 mg/mL each), 200 mcg dose = 5 units (0.05 mL). For separate 10 mg vials: add 3.0 mL BAC water to each = 3.33 mg/mL, 200 mcg dose = 6 units (0.06 mL). Step-by-step: inspect the vial; wipe stopper with alcohol prep pad and let dry; draw planned BAC water volume with a sterile syringe; inject BAC water slowly down the inside wall of the vial (do not spray directly onto powder); gently swirl (do not shake) until powder dissolves; label with date and concentration; refrigerate at 35.6-46.4°F (2-8°C) shielded from light; use within 28-30 days; discard if cloudy, discolored, or contaminated.
Reported by
peptidedosingprotocols.com
What it saysHide(secondary reference; provider confirmation required). [Source: peptidedosingprotocols.com]
CJC-1295 / Ipamorelin (No Dac)
Recovery
200-300 mcg of each peptide daily, typically administered once daily SubQ.
Frequency
not stated
Reconstitution
Add 3.0 mL BAC water to a 20 mg blend vial.
Reported by
Peptide Dosing Protocols stack
What it saysHide(secondary reference; provider confirmation required).
DSIP
Sleep
Standard: 100-200 mcg · Low: 100 mcg · High: 300 mcgSubQ
Frequency
once daily
Duration
5-7 nights
Cycle
short course
Reconstitution
2.0 mL bacteriostatic water
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). The standard dosing for DSIP is 100-200 mcg subcutaneously once daily.
100-300 mcg subcutaneous; 25 nmol/kg IV (~1.5 mg for 70 kg person)subcutaneous or intranasal
Frequency
Once daily before bed; or every 2-3 days
Duration
have not been established.
Reconstitution
Bacteriostatic water
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Epitalon
Longevity · Sleep · Cognitive Support
Standard: 5 mg/day · High: 10 mg/daySubQ
Frequency
2-3 times per year
Duration
20 days
Cycle
short cycle
Reconstitution
Add 2.0 mL BAC water to a 10 mg vial.
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: half-life of epitalon? Epitalon clears the bloodstream very quickly, on the order of minutes, whic. The standard dosing for Epitalon is 5 mg per day for 20 days.
5-10 mg per daysubcutaneous or oral
Frequency
Once daily
Reconstitution
Bacteriostatic water
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Referenced at 10 mg daily for 10 days followed by a break; longevity stacking contexts cite 2-3 cycles per year. General dosage guideline of 5-10 mg per cycle also referenced.subcutaneous or nasal
Frequency
Daily during 10-day cycle
Timing
Evening cited for sleep-phase cellular repair; morning for circadian alignment
Cycle
10-day cycles, 2-3 per year
Reported by
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
FOXO4-DRI
Recovery
Standard: 250 mcg daily · Low: 250 mcg daily · High: 500 mcg dailySubQ
Frequency
daily
Duration
16 weeks
Cycle
4 weeks on, 4 weeks off
Reconstitution
3.0 mL BAC water
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Standard dosing starts at 250 mcg daily and increases to 500 mcg daily.
GHK-Cu
Tissue Repair · Inflammation · Longevity · Recovery · Post-Procedure Recovery
Standard: 1.0-1.5 mg · Low: 1.0 mg · High: 2.0 mgSubcutaneous injection
Frequency
Daily
Duration
4-6 weeks
Cycle
4-8 weeks
Reconstitution
50 mg vial reconstituted with 2.0 mL bacteriostatic water
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). The standard dosing for GHK-Cu is 1.0-1.5 mg daily for 4-6 weeks.
1-2 mg SC daily, or topical applicationsubcutaneous or topical
Frequency
once daily
Duration
30-day cycles
Vial
Commonly 50 mg vials (injectable) or topical formulations
Reconstitution
Reconstitute with bacteriostatic water (injectable)
Reported by
Commonly published practitioner dosing range - provider confirmation required.
Topical: 0.01-1% in cream/serum; SC injection: 1-2 mg dailysubcutaneous or oral or topical
Frequency
Topical: once or twice daily; SC: once daily
Cycle
Repeatable
Reconstitution
Bacteriostatic water
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Topical: serum to face and neck twice daily (AM and PM) after cleansing, before moisturizer. Injectable referenced at 200-300 mcg (0.2-0.3 mg) before bedtime in recovery contexts; sterile technique and supervision stressed. Microneedling before topical cited to enhance absorption.topical or subcutaneous
Frequency
Topical 2x daily; injectable nightly in recovery contexts
Timing
Topical AM/PM; injectable before bed
Reported by
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
GHRP-6
Recovery
Standard: 300 mcg · Low: 100 mcg · High: 300 mcgSubQ
Frequency
3x daily, at least 4 hours apart
Duration
null
Cycle
8 weeks on, 4 weeks off
Reconstitution
3 mL bacteriostatic water
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: null. GHRP-6 is typically dosed at 100-300 mcg per injection.
Cycling reference: 6-8 weeks on, 4 weeks off. Cited for appetite stimulation and GH release with fat metabolism support.subcutaneous
Frequency
not stated
Cycle
On-cycle 6-8 weeks, off-cycle 4 weeks
Reported by
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
GLOW
Tissue Repair · Skin & tissue · Soft Tissue Recovery · Recovery
Pre-blend: 0.10 mL (10 units) daily = ~2.33 mg total blend (~1.67 mg GHK-Cu, ~0.33 mg TB-500, ~0.33 mg BPC-157). Separate vials: BPC-157 250-500 mcg/day; TB-500 2.5-5 mg per injection 2x/week (loading), 2 mg per injection (maintenance); GHK-Cu 1-2 mg/day (loading), 1 mg/day (maintenance).subcutaneous
Frequency
Once daily (pre-blend); separate vials: BPC-157 daily, TB-500 twice weekly, GHK-Cu daily
Duration
Standard: 4-6 weeks on, 2-4 weeks off; Extended: 8-12 weeks on, 4-8 weeks off; Skin-focused blend: 4 weeks on, 2-4 weeks off. GHK-Cu copper cycling typically governs cycle length: 30-60 days on, equal time off.
Reconstitution
Pre-blend (70 mg vial): Add 3.0 mL bacteriostatic water → ~23.3 mg/mL total blend; standard daily dose is 0.10 mL (10 units) = ~2.33 mg total. Separate vials, BPC-157 5 mg vial: add 2 mL BAC water → 2,500 mcg/mL; BPC-157 10 mg vial: add 2 mL BAC water → 5,000 mcg/mL. TB-500 5 mg vial: add 1 mL BAC water → 5 mg/mL; TB-500 10 mg vial: add 2 mL BAC water → 5 mg/mL. GHK-Cu 50 mg vial: add 5 mL BAC water → 10 mg/mL; GHK-Cu 100 mg vial: add 10 mL BAC water → 10 mg/mL. Standard steps: wipe stopper with alcohol swab and let dry; draw planned BAC water volume; aim water at inside of glass wall, not directly onto powder; let water flow slowly down vial wall; roll gently 30-60 seconds until dissolved (do not shake); inspect for clarity (GHK-Cu may show slight blue/green tint from copper; BPC-157 and TB-500 should be clear); label with reconstitution date and store at 2-8 °C (35.6-46.4 °F). TB-500 is the limiting reconstitution-stability window, most workflows use the reconstituted blend within 1-2 weeks.
Reported by
peptidedosingprotocols.com
What it saysHide(secondary reference; provider confirmation required). [Source: peptidedosingprotocols.com]
10 units (0.10 mL) daily, approximately 2.33 mg total blend.
Frequency
not stated
Reconstitution
Add 3.0 mL bacteriostatic water to reconstitute.
Reported by
Peptide Dosing Protocols stack
What it saysHide(secondary reference; provider confirmation required).
Glutathione
Immune Support · Inflammation · Longevity · Metabolic Health · Recovery
Standard: 250-1,000 mg/day · Low: 250 mg/day · High: 1,000 mg/dayOral
Frequency
daily
Duration
4 weeks - 6 months
Cycle
4 weeks or longer to appear and faded after stopping.
Reconstitution
reconstitution and supplies for injectable use, side effects, and where the evidence stops.
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Standard oral dosing ranges from 250 to 1,000 mg per day.
200-600 mg per injection (IM/SC); 600-1400 mg per session (IV push); 500-1000 mg daily (oral)subcutaneous or intranasal or oral or topical or intramuscular
Frequency
1-3 times per week (injectable); daily (oral)
Duration
Key limitations / notes
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
HCG
Recovery
250-500 IU per injection (TRT adjunct); 1500-5000 IU (fertility protocols)subcutaneous or oral or topical or intramuscular
Frequency
Every other day or 2-3 times per week
Reconstitution
Bacteriostatic water
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Humanin
Recovery
Standard: 4 mg/kg · Low: 2.5 mg/kg/injectionIP
Frequency
2x/week
Duration
4-12 weeks
Cycle
6-8 weeks
Reconstitution
reconstituted with 2 mL bacteriostatic water (10 mg/mL)
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). The standard dosing for Humanin is 4 mg/kg administered via intraperitoneal injection twice weekly.
IGF-1 LR3
Recovery
Standard: 40 mcg/day · Low: 20 mcg/day · High: 100 mcg/daySubcutaneous (SubQ) or intramuscular (IM) injection
Frequency
Once daily
Duration
4 weeks
Cycle
4 weeks on, 4 weeks off
Reconstitution
reconstituted in 2 mL of bacteriostatic water (500 mcg/mL)
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: 20-30 hours. Standard dosing is 40 mcg/day.
Ipamorelin
Sleep · Body Composition · Recovery · Performance Recovery · Longevity
Standard: 200-300 mcg/day · Low: 100 mcg/day · High: 300 mcgsubcutaneous
Frequency
1-3 times per day
Duration
8-12 weeks on, then 4 weeks off
Cycle
8-12 weeks
Reconstitution
3.0 mL BAC water for 10 mg vial
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: about two hours. Most research protocols use 100 to 300 mcg per shot, one to three times per day.
200-300 mcg per injectionsubcutaneous
Frequency
not stated
Duration
8-12 weeks, then 2-4 weeks off
Cycle
Repeatable
Reconstitution
Bacteriostatic water
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Referenced at 100-300 mcg (0.1-0.3 mg) per dose. Commonly paired with CJC-1295 for synergistic GH release with minimal cortisol or prolactin elevation. Sample stacks cite 100 mcg each, fasted morning and before bed.subcutaneous
Frequency
2-3x daily (vitality contexts cite once daily)
Timing
Morning fasted, pre-workout, or before bed
Cycle
On-cycle 8-12 weeks, off-cycle 4 weeks
Reported by
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
Kisspeptin-10
Recovery
Standard: 100-200 mcg · Low: 50-100 mcgSubcutaneous (SC)
Frequency
Once daily SC
Cycle
8 weeks: 56 doses needed; one vial still leaves margin for losses and schedule adjustments Insulin Syringes (U-100, 0.
Reconstitution
Reconstitute with bacteriostatic water (BAC water) before drawing any dose.
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: about 4 minutes. The standard dosing for Kisspeptin-10 is 100-200 mcg once daily.
9.6 nmol/kg SC (IVF trigger); 1 nmol/kg IV (diagnostic challenge)subcutaneous
Frequency
Single dose (IVF trigger); single dose (diagnostic)
Duration
Single administration per IVF
Cycle
Repeatable
Reconstitution
Sterile water
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
KLOW
Tissue Repair · Skin & tissue · Gut Health · Inflammation · Recovery
~6.0 mg total blend per day (22.5 units / 0.225 mL at 3.0 mL reconstitution) at maintenance phase; titration starts at ~2.0 mg total blend (7.5 units) and steps up to ~4.0 mg (15 units) then ~6.0 mg (22.5 units)subcutaneous
Frequency
Once daily
Duration
Standard: 4-6 weeks on, 2-4 weeks off; Extended: 8-12 weeks on, 4 weeks off; Maximum extension: up to 16 weeks on, 4+ weeks off (no safety data supports continuous use beyond 12 weeks)
Reconstitution
Add 3.0 mL bacteriostatic water to the 80 mg vial to yield ~26.7 mg/mL total blend concentration. Steps: (1) Inspect the vial, check label, batch, and powder appearance; a discolored or damp vial should not be used. (2) Sanitize, swab the rubber stopper of the KLOW vial and the bacteriostatic water vial with alcohol prep pads and let dry. (3) Draw 3.0 mL bacteriostatic water with a sterile syringe; vent the BAC water vial if needed. (4) Inject down the vial wall, insert the needle and inject BAC water slowly down the inside wall; avoid spraying directly onto the powder to prevent foaming and denaturation. (5) Swirl, do not shake, gently swirl or roll until powder is fully dissolved; solution should be clear with possible light blue tint from GHK-Cu (normal). (6) Label with reconstitution date and store at 35.6-46.4°F (2-8°C), protected from light. (7) Use within 14-28 days when refrigerated; avoid freeze-thaw cycles.
Reported by
peptidedosingprotocols.com
What it saysHide(secondary reference; provider confirmation required). [Source: peptidedosingprotocols.com]
Commonly, a once-daily subcutaneous injection is used, with a typical cycle of 4-6 weeks on and 2-4 weeks off.
Frequency
not stated
Reconstitution
Add 3.0 mL of bacteriostatic water to the 80 mg vial.
Reported by
Peptide Dosing Protocols stack
What it saysHide(secondary reference; provider confirmation required).
KPV
Inflammation · Gut Health · Tissue Repair · Recovery
Standard: 200-500 mcg once or twice daily · Low: 200 mcg/day SubQ · High: 500 mcg twice dailyoral, subcutaneous
Frequency
once or twice daily
Duration
Weeks 1-8+
Cycle
4-8 weeks on, 2-4 weeks off
Reconstitution
2 mL BAC water
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). KPV is commonly dosed at 200-500 mcg once or twice daily for gut health.
200-500 mcg SC daily, or 250-500 mcg orally twice daily for gut-directed supportsubcutaneous or oral
Frequency
once daily (SC) or twice daily (oral)
Duration
4-8 weeks
Cycle
4-8 week cycles
Vial
Commonly 5 mg vials (injectable) or oral capsules
Reconstitution
Reconstitute with bacteriostatic water (injectable form)
Reported by
Commonly published practitioner dosing range - provider confirmation required.
200-500 mcg per injection (SC); or oral capsules as directedsubcutaneous or oral
Frequency
Once daily (SC); 1-2 times daily (oral)
Reconstitution
Bacteriostatic water
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
LL-37
Immune Support · Inflammation · Gut Health · Tissue Repair
100 mcg SC daily or every other daysubcutaneous
Frequency
daily or every other day
Duration
2-4 weeks
Cycle
Short cycles; reassess
Vial
Commonly 5 mg vials
Reconstitution
Reconstitute with bacteriostatic water
Reported by
Commonly published practitioner dosing range - provider confirmation required.
100-200 mcg daily (SC); topical per formulationsubcutaneous or oral or topical
Frequency
Once daily, 5 days per week (SC); as directed (topical)
Duration
2-4 weeks, then 2 weeks off
Cycle
Repeatable
Reconstitution
Bacteriostatic water
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Mazdutide
Metabolic Health · Body Composition · Weight Management · Appetite Regulation
2.5-5 mgsubcutaneous
Frequency
Once weekly
Duration
Titrate over weeks per tolerance
Vial
10 mg vial
Reconstitution
10 mg vial + 3.0 mL bacteriostatic water (~3.33 mg/mL). Draw 3.0 mL BAC water, inject slowly down the vial wall, swirl gently (do not shake), refrigerate at 2-8 C protected from light.
Reported by
Peptide Dosages -
What it saysHide(secondary reference; provider confirmation required). Educational protocol, mirrors clinical-trial gradual titration. [Source: Peptide Dosages]
Melanotan I (MT-1)
Recovery
0.16 mg/kg daily (injection protocol); 16 mg implant every 2 months (approved)subcutaneous or oral
Frequency
Daily for 10 days per
Duration
10-day injection
Reconstitution
Sterile water
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Melanotan II (MT-2)
Recovery
Loading: 0.25-0.5 mg daily; Maintenance: 0.5-1.0 mg 1-2 times per weeksubcutaneous or oral
Frequency
Daily during loading (1-3 weeks); 1-2 times per week for maintenance
Duration
Loading: 2-4 weeks; Maintenance: ongoing as desired
Reconstitution
Bacteriostatic water
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Standard: 500-1000 mcg daily · Low: 100-250 mcg daily · High: 250-500 mcg dailySubcutaneous (SubQ) injection
Frequency
1-2x per week
Duration
4-6 weeks
Cycle
6 weeks
Reconstitution
2 mL BAC water
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: half-life Melanotan 2 ~1-2 hours Afamelanotide(MT-I) ~0. The standard loading dose is 500-1000 mcg daily.
Methylene Blue
Recovery
Standard: 1 mg/kg · Low: 5-20 mg/day · High: 10 mg/kgIV, Oral
Frequency
not stated
Reconstitution
no
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Methylene blue is typically dosed at 1 mg/kg for methemoglobinemia.
MOTS-c
Metabolic Health · Longevity · Performance Recovery · Recovery
Standard: 5 mg · Low: 5 mg · High: 10 mgSubcutaneous injection
Frequency
2 to 3 times per week
Duration
4 to 8 weeks
Cycle
4-6 weeks
Reconstitution
Reconstitute a 10 mg vial with 1.0 mL bacteriostatic water
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). The standard dosing is 5 mg subcutaneously, 3 times per week for 4-6 weeks.
5-10 mg SC 2-3 times weeklysubcutaneous
Frequency
2-3 times weekly
Duration
4 weeks
Cycle
4-week cycles
Vial
Commonly 10 mg vials
Reconstitution
Reconstitute with bacteriostatic water
Reported by
Commonly published practitioner dosing range - provider confirmation required.
5-10 mg per injectionsubcutaneous
Frequency
2-3 times per week or every 5 days
Reconstitution
Bacteriostatic water
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Referenced at 10 mg weekly in divided doses within longevity stacking. Morning administration cited for circadian and mitochondrial alignment.subcutaneous
Frequency
Weekly total (10 mg), divided doses
Timing
Morning
Cycle
Cycle every few months in longevity contexts
Reported by
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
NAD+
Longevity · Metabolic Health · Cognitive Support · Recovery
Standard: 100 mg · Low: 50 mg · High: 200 mgSubQ
Frequency
2-3x weekly
Duration
4-8 weeks
Cycle
4 weeks, then escalate to 100 mg weekly.
Reconstitution
7.5 mL BAC water for 750 mg vial
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). The standard dose for NAD+ is 100 mg administered 2-3 times weekly.
250-750 mg per IV session; 300-1000 mg daily for oral precursors (NR/NMN)oral
Frequency
1-2x weekly IV (loading), monthly (maintenance); daily oral
Duration
4-8 week loading phase IV; ongoing for oral precursors
Reconstitution
Normal saline (0.9% NaCl) 250-500 mL for IV
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Oxytocin Acetate
Recovery
Standard: 200-300 mcg · Low: 100 mcg · High: 400-500 mcgSubcutaneous
Frequency
Once daily
Cycle
cycle Duration 1-2 weeks Review Point Week 2 Notes Used in mood, anxiety, and pain research blocks.
Reconstitution
add 2-3 mL bacteriostatic water to a 2 mg or 5 mg lyophilized vial
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: about 3-6 minutes. The standard dosing for subcutaneous oxytocin is 200-300 mcg once daily.
24 IU intranasal (6 sprays of 4 IU each, 3 per nostril)intranasal
Frequency
Once or twice daily for research protocols
Cycle
nonapeptide neurohormone produced in the h
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
P21
Recovery
Practitioner ranges vary; commonly ~1 mg SC daily - provider-directedsubcutaneous
Frequency
once daily
Duration
Per provider direction
Vial
Compounded; varies
Reconstitution
Reconstitute with bacteriostatic water
Reported by
Commonly published practitioner dosing range - provider confirmation required.
Pinealon
Recovery
Standard: 1.0 to 2.0 mg per day · Low: 1.0 mg · High: 2.0 mgsubcutaneous injection or oral
Frequency
Once daily
Duration
10 to 20 days
Cycle
2 to 3 cycles per year
Reconstitution
3.0 mL BAC water
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Pinealon is typically dosed at 1.0 to 2.0 mg per day.
PT-141
Recovery
Standard: 1.75 mgSubcutaneous (abdomen or thigh)
Frequency
1 dose per 24 hours
Cycle
8 doses per month
Reconstitution
reconstitution math, route considerations, and side effects for this bremelanotide peptide.
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). The standard dose of PT-141 is 1.75 mg subcutaneous at least 45 minutes before sexual activity.
1.75 mgsubcutaneous or oral
Frequency
not stated
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Injection referenced at 0.5-2 mg per dose with onset in 1-4 hours, peak at 6-12 hours, duration 24-72 hours. Nasal spray referenced at 2-4 sprays per nostril, onset 30-60 minutes, duration 6-12 hours. Start low; side effects are dose-dependent (nausea most common).subcutaneous or nasal
Frequency
As needed
Timing
On-demand, 1-4 hours before desired effect (injection)
Reported by
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
Retatrutide
Metabolic Health · Body Composition · Weight Management · Appetite Regulation
Standard: 1 mg titrating to 12 mg once weekly (Phase 3 TRIUMPH: 1 mg → 2 mg → 4 mg → 6 mg → 9 mg → 12 mg, stepping up every 4 weeks) · Low: 1 mg once weekly · High: 12 mg once weeklySubcutaneous injection
Frequency
Once per week, on the same day each week
Duration
48 weeks (Phase 2 reference); 68-72 weeks (Phase 3 TRIUMPH reference)
Cycle
Every 4 weeks at each dose step; review at 4 mg, 9 mg, 12 mg; slow titration option adds 2-4 weeks per dose step when GI symptoms persist
Reconstitution
10 mg vial with 2.0 mL BAC water yields a concentration of 5 mg/mL; use U-100 insulin syringes
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Retatrutide is dosed once weekly via subcutaneous injection starting at 1 mg and titrating up every 4 weeks to a maximum of 12 mg following the Phase 3 TRIUMPH schedule.
1-12 mgsubcutaneous
Frequency
Once weekly
Timing
Any time of day
Duration
Titrate over weeks per response/tolerance
Reported by
Reference titration range, provider confirm
Retatrutide / MOTS-c
Metabolic Health · Body Composition · Weight Management · Mitochondrial Function
Retatrutide: titrate 1 → 2 → 4 → 6 → 9 mg weekly (maintenance 9 mg or 12 mg weekly); MOTS-c intermittent: 5 mg 2-3x weekly (maintenance 5-10 mg 2-3x weekly); MOTS-c low-dose daily alternative: start 200 mcg/day up to 1 mg/daysubcutaneous
Frequency
Retatrutide: once weekly; MOTS-c: 2-3x weekly (intermittent) or daily (low-dose alternative)
Duration
Conservative starter: retatrutide weeks 1-24, MOTS-c weeks 5-12 then off; Standard research cycle: retatrutide weeks 1-48, MOTS-c 8-week pulses with 4-week breaks; Aggressive deficit: retatrutide weeks 1-68, MOTS-c 6 weeks on / 2 weeks off repeating
Reconstitution
Standard 7-Step Reconstitution: (1) Let the lyophilized vial and BAC water reach room temperature before opening. (2) Swab both vial stoppers with alcohol and let them dry. (3) Pull the planned BAC water volume into a sterile syringe. (4) Inject the BAC water slowly down the inside of the vial wall, do not aim at the powder. (5) Swirl or roll gently until fully dissolved; do not shake. (6) Confirm the solution is clear; label the vial with compound name, concentration, and date. (7) Store at 2-8 °C; use retatrutide within 28-60 days and MOTS-c within 7 days for best potency. Each compound is reconstituted separately. Retatrutide common concentration: 10 mg vial + 1.0 mL BAC water = 10 mg/mL. MOTS-c common concentration: 10 mg vial + 2.0 mL BAC water = 5 mg/mL. MOTS-c aliquots may be stored at -20 °C for 2-3 months.
Reported by
peptidedosingprotocols.com
What it saysHide(secondary reference; provider confirmation required). [Source: peptidedosingprotocols.com]
RT3
Recovery
Standard: 1 mg once weekly · Low: 1 mg · High: 12 mgSubcutaneous injection
Frequency
once per week
Duration
null
Reconstitution
null
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: null. The standard dosing starts at 1 mg once weekly and titrates up to 12 mg.
0.5 mg starting dose, escalating to target of 8-12 mgsubcutaneous or oral
Frequency
Once weekly
Duration
Key Question
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Russian Nootropic Stack
Cognitive Function · Longevity · Neuroprotection
Semax: 300-800 mcg/day (SubQ), 400-600 mcg/day (intranasal); Selank: 250-500 mcg/day (SubQ), 400-750 mcg/day (intranasal). Maintenance example: Semax 600 mcg + Selank 400 mcg daily.Subcutaneous injection or intranasal (drops/spray)
Frequency
Once daily (SubQ, morning before 2 PM); 2x daily for Semax intranasal (AM + early afternoon); 2-3x daily for Selank intranasal
Duration
Standard cycle: 4 weeks on / 4 weeks off; Short intensive: 10-14 days on / 10-14 days off; Extended: 8 weeks (5 days/week) / 4 weeks off; Clinical intranasal reference: 14-21 days on / 14-21 days off
Reconstitution
Each compound is reconstituted separately using bacteriostatic water (BAC water). For a 10 mg vial: add 3.0 mL BAC water to reach 3,333 mcg/mL, or 2.0 mL to reach 5,000 mcg/mL. For a 5 mg vial: add 2.0 mL BAC water to reach 2,500 mcg/mL, or 1.0 mL to reach 5,000 mcg/mL. Use 0.3 mL / 30-unit insulin syringes for accuracy at small volumes. Label each vial with compound name, concentration, and reconstitution date. Semax is light-sensitive (contains methionine, which oxidizes when exposed to light); wrap the reconstituted vial in aluminum foil or store in a dark container. Both vials are stable for approximately 30 days at 2-8 °C once reconstituted.
Reported by
peptidedosingprotocols.com
What it saysHide(secondary reference; provider confirmation required). [Source: peptidedosingprotocols.com]
Selank
Neuroinflammation · Sleep
Standard: 250-500 mcg per day · Low: 250 mcg · High: 500 mcgSubcutaneous injection (SubQ) or intranasal nasal spray
Frequency
once daily for SubQ; 14-21 days for intranasal
Duration
4 weeks for SubQ; 14-21 days for intranasal
Cycle
4 weeks on, 4 weeks off for SubQ; 1-3 weeks off for intranasal
Reconstitution
3 mL, 6 mL, or 10 mL per 30 mg vial
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: Half-life Selank ~2-10 min plasma; 12-24 hr functional duration Semax ~3-5 min plasma; 20-24. The standard research-use SubQ schedule is 4 weeks on at 250-500 mcg per day.
250-500 mcg intranasally daily (or 0.15% solution per protocol)intranasal
Frequency
once or twice daily
Duration
10-14 day cycles
Cycle
Repeat cycles per provider direction
Vial
Compounded intranasal solution/spray
Reconstitution
Compounded intranasal solution - no reconstitution required
Reported by
Commonly published practitioner dosing range - provider confirmation required.
300-600 mcg per dose intranasal; 200-400 mcg per dose subcutaneoussubcutaneous or intranasal or oral
Frequency
2-3 times daily (intranasal); once daily (SC, 5 days per week)
Duration
30 days on, 30 days off; approximately 6
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Referenced at 250-500 mcg (0.25-0.5 mg) per dose. Nasal spray cited as the most common route; onset within 30-60 minutes, peak benefit after several days of consistent use.nasal or subcutaneous
Frequency
Nasal 1-3x daily; injection once daily or as needed
Reported by
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
Semaglutide
Metabolic Health · Body Composition · Weight Management · Appetite Regulation
Standard: 2.4 mg · Low: 0.25 mg · High: 2.4 mgsubcutaneous injection
Frequency
once weekly
Duration
16 weeks
Cycle
16 weeks on, ongoing weekly maintenance
Reconstitution
reconstituted lyophilized vial
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: about 7 days. Standard dosing escalates to 2.4 mg over 16 weeks.
Semax
Neuroinflammation
Standard: 0.6 to 1.5 mg/day · Low: sub-milligram per administration · High: up to ~1 mg/kgIntranasal solution, Subcutaneous injection
Frequency
Daily administration, often divided into morning and afternoon doses
Duration
10-14 days for standard short course, 4-6 weeks for extended cognitive course
Cycle
4-6 weeks on, 2-4 weeks off
Reconstitution
Requires reconstitution with bacteriostatic water before subcutaneous research use
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Standard dosing for Semax is about 0.6 to 1.5 mg per day, typically split into two administrations.
300-600 mcg intranasally daily (or 1-3 drops/nostril of 0.1% solution)intranasal
Frequency
once or twice daily
Duration
10-14 day cycles
Cycle
Repeat cycles per provider direction
Vial
Compounded intranasal solution/spray
Reconstitution
Compounded intranasal solution - no reconstitution required
Reported by
Commonly published practitioner dosing range - provider confirmation required.
200-600 mcg per dose (0.1% solution) for nootropic use; 3-6 mg per dose (1% solution) for strokeintranasal
Frequency
2-3 times daily
Cycle
Repeatable
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Referenced at 300-600 mcg (0.3-0.6 mg) daily in brain health protocols. Morning administration cited to carry cognitive benefit through the day. Rotation with other nootropic peptides between cycles referenced to maintain response.nasal
Frequency
Daily
Timing
Morning
Cycle
4 weeks on with rotation between cycles
Reported by
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
Sermorelin Acetate
Longevity · Sleep · Body Composition · Metabolic Health · Recovery
Standard: 200-300 mcg · Low: 100 mcg · High: 400-500 mcgsubcutaneous injection
Frequency
nightly
Duration
3-6 months
Cycle
Cycle length Planning note 4 weeks 1 vial 28 nights of 200 mcg from one vial.
Reconstitution
3 mL bacteriostatic (BAC) water in a 10 mg vial gives 3 units per 100 mcg on a U-100 insulin syringe.
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: 11-12 minutes. Common dose is 200-300 mcg subQ nightly.
200-300 mcgsubcutaneous or oral
Frequency
Once daily at bedtime, 5 nights per week
Duration
3-6 months
Reconstitution
Bacteriostatic water
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
SLU-PP-322
Recovery
Standard: 50 mg (1 tablet) · Low: ≤1.5 mg · High: 100 mg (2 tablets)oral
Frequency
once daily
Cycle
6 weeks IP dosing (Burris-lab standard) Improved ejection fraction; reduced fibrosis; increased survival in the TAC model.
Reconstitution
5 mg vial reconstituted with 2 mL BAC water (2,500 mcg/mL) and a daily 500 mcg subcutaneous draw, a common community pattern, not a clinically validated dose.
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: half-life, there is no validated missed-dose rule. The standard dosing for SLU-PP-332 is 50 mg taken once daily.
SNAP-8
Recovery
3-10% concentration in formulation (solution, not pure peptide)topical
Frequency
Twice daily (morning and evening)
Duration
Continuous use; minimum 28 days for measurable results
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
SS-31
Mitochondria
40 mg subcutaneous daily (Phase 3 dose); or 5-10 mg daily (research community range)subcutaneous
Frequency
Once daily
Cycle
Repeatable
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Standard: 40 mg · Low: 20 mgSubcutaneous
Frequency
once daily
Cycle
12 weeks per period Open-label extension All remaining patients on elamipretide 40 mg SC daily Up to 168 weeks Reported in Genetics in Medicine, 2024.
Reconstitution
reconstitution, and safety considerations for this mitochondria-targeted peptide.
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). The standard dosing for SS-31 is 40 mg subcutaneously once daily.
Investigational; commonly studied at 40 mg SC daily - provider-directedsubcutaneous
Frequency
once daily (study protocols)
Duration
Per provider/study protocol
Vial
Compounded; varies
Reconstitution
Reconstitute per compounding pharmacy instructions
Reported by
Commonly published practitioner dosing range - provider confirmation required.
Survodutide
Recovery
Standard: 4.8 mg once weekly · Low: 0.6 mg once weekly · High: 4.8 mg once weeklySubcutaneous injection
Frequency
once weekly
Duration
76 weeks
Cycle
null
Reconstitution
1.0 to 2.0 mL per vial
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: null. Survodutide is administered at a standard dose of 4.8 mg once weekly.
TA1
Immune Support · Inflammation · Recovery · Post-Procedure Recovery
Standard: 1.6 mgSubcutaneous
Frequency
Twice weekly
Duration
6 to 12 months
Cycle
12 weeks Community-derived, not from a clinical trial These durations are reported from research, not personal-use recommendations.
Reconstitution
2 mL bacteriostatic water
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: half-life of about 2-3 hours. The standard dosing for TA1 is 1.6 mg subcutaneously twice weekly.
1.5 mg SC twice weekly, or 450-900 mcg dailysubcutaneous
Frequency
twice weekly, or daily
Duration
4-12 weeks
Cycle
Repeat course per provider direction
Vial
Commonly 5 mg or 10 mg vials
Reconstitution
Reconstitute with sterile/bacteriostatic water
Reported by
Commonly published practitioner dosing range - provider confirmation required.
1.6 mgsubcutaneous or oral
Frequency
Twice weekly (standard); daily during chemotherapy
Reconstitution
Sterile water
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Cycling reference for immune support: 6-12 weeks on, 6 weeks off.subcutaneous
Frequency
not stated
Cycle
On-cycle 6-12 weeks, off-cycle 6 weeks
Reported by
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
TB-500 / TB4
Tissue Repair · Soft Tissue Recovery · Tendon/Ligament Recovery · Injury Recovery · Performance Recovery · Recovery
Standard: 2-2.5 mg subcutaneous twice weekly · Low: 2 mg twice weekly · High: 2.5 mg subcutaneous once weeklysubcutaneous injection
Frequency
twice weekly for loading, once weekly for maintenance
Duration
Weeks 1-6 for loading, Weeks 7-12 for maintenance
Cycle
4-12 weeks
Reconstitution
2.0 mL bacteriostatic water for 10 mg vial
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). TB-500 is commonly dosed at 2-2.5 mg subcutaneously twice weekly during the loading phase.
2-2.5 mg SC twice weekly (loading 4-6 weeks), then 2 mg weekly maintenancesubcutaneous
Frequency
twice weekly (loading), then weekly
Duration
4-6 week loading, then maintenance
Cycle
Loading phase then maintenance phase
Vial
Commonly 5 mg or 10 mg vials
Reconstitution
Reconstitute with bacteriostatic water
Reported by
Commonly published practitioner dosing range - provider confirmation required.
2-2.5 mg per injection (loading); 750 mcg-2 mg (maintenance)subcutaneous or oral or topical
Frequency
not stated
Reconstitution
Bacteriostatic water
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Referenced with a loading phase of 5-10 mg weekly for 4-6 weeks, then maintenance at 2-5 mg weekly split into smaller doses. Results typically noticed within 2-4 weeks. Caution flag: angiogenesis-promoting, so cancer or tumor history warrants medical consultation first.subcutaneous or intramuscular
Frequency
Weekly total (2-10 mg) split into smaller doses
Timing
Post-workout or before bed
Cycle
On-cycle 4-6 weeks, off-cycle 1-2 months
Reported by
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
Tesamorelin
Metabolic Health · Body Composition · Cognitive Support
Standard: 2 mg once a daySubcutaneous injection in the abdomen only
Frequency
Once daily
Duration
26 weeks
Cycle
Cycle length Planning note 4 weeks 6 vials (10 mg) 28 daily doses needed.
Reconstitution
Reconstitute with bacteriostatic water
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: Half-Life Tesamorelin 26-38 minutes Sermorelin 11-12 minutes CJC-1295(DAC) ~6-8 days Fe. The standard dosing for Tesamorelin is 2 mg administered once daily.
1-2 mg SC daily at bedtimesubcutaneous
Frequency
once daily at bedtime
Duration
12+ weeks
Cycle
Continuous per provider direction
Vial
Commonly 2 mg vials
Reconstitution
Reconstitute with sterile/bacteriostatic water
Reported by
Commonly published practitioner dosing range - provider confirmation required.
2 mgsubcutaneous
Frequency
Once daily
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Fat loss stacking reference: 200 mcg (0.2 mg) fasted morning paired with AOD-9604 300 mcg. Cited for visceral fat targeting with Mod GRF 1-29 as a pulse-enhancing partner.subcutaneous
Frequency
Daily in stack contexts
Timing
Morning, fasted
Reported by
Peptide Therapy Reference Text, internal KB v4
What it saysHide(Aug 2026), secondary reference; provider confirmation required.
Tesamorelin / Ipamorelin
Body Composition · Metabolic Health · Recovery · Longevity
Tesamorelin 1-2 mg + Ipamorelin 200-300 mcg (separate vials); or blend draws of 10-40 units/day depending on vial ratio (5/5 vial example: up to 667 mcg tesamorelin + 667 mcg ipamorelin at 40 units)subcutaneous
Frequency
Once daily subcutaneous
Duration
Standard: 8-12 weeks on, 4-8 weeks off; Extended: 12-16 weeks on, 4-8 weeks off; typical cycle 8-16 weeks
Reconstitution
Add 3.0 mL bacteriostatic water to the 3 mL vial, aiming the stream down the inside vial wall; swirl gently (do not shake) until fully dissolved. Inspect: solution should be clear; discard if cloudy or full of particles. Label with ratio, date, and concentration. Refrigerate at 2-8°C (35.6-46.4°F), protected from light. 10/3 vial yields ~4.33 mg/mL total (3.33 mg/mL tesamorelin + 1.0 mg/mL ipamorelin); 5/5 vial yields ~3.33 mg/mL total (1.67 mg/mL each). Do not freeze reconstituted solution.
Reported by
peptidedosingprotocols.com
What it saysHide(secondary reference; provider confirmation required). [Source: peptidedosingprotocols.com]
Tesofensine
Recovery
Standard: 0.5 mg once daily · Low: 0.25 mg once dailyOral tablet
Frequency
once daily
Duration
24 weeks
Cycle
24 weeks on
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: about 9 days. The standard dosing for tesofensine is 0.5 mg taken once daily.
Tirzepatide
Metabolic Health · Body Composition · Weight Management · Appetite Regulation
2.5-15 mgsubcutaneous
Frequency
Once weekly
Timing
Any time of day
Duration
Titrate over weeks per response/tolerance
Reported by
Reference titration range, provider confirm
Standard: 5 mg, 10 mg, or 15 mg once weekly (three approved maintenance doses) · Low: 2.5 mg once weekly · High: 15 mg once weeklySubcutaneous injection
Frequency
Once weekly
Duration
Weeks 1-4 at 2.5 mg, stepping up every 4 weeks; full ladder to 15 mg reached at week 21+
Cycle
4 weeks (Phase 1, 2.5 mg), 8 weeks (through 5 mg), 12 weeks (through 7.5 mg), 16 weeks (through 10 mg), 24 weeks (full ladder to 15 mg)
Reconstitution
40 mg vial + 2.0 mL BAC water = 20 mg/mL; reconstituted solution keeps for ~28 days refrigerated
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: 5 days. Tirzepatide is titrated once weekly from 2.5 mg up to a maximum of 15 mg in 2.5 mg increments every 4 weeks via subcutaneous injection.
TRZ
Recovery
Standard: 5 mg once weekly · Low: 2.5 mg once weekly · High: 15 mg once weeklySubcutaneous injection
Frequency
once weekly
Cycle
Cycle length Planning note 4 weeks (Phase 1, 2.
Reconstitution
2.0 mL BAC water for a 40 mg vial
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: 5 days. Tirzepatide is administered once weekly, starting at 5 mg.
2.5 mg starting dose, escalating to 5-15 mg maintenancesubcutaneous or oral
Frequency
Once weekly
Duration
Ongoing (chronic therapy)
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
VIP
Immune · Neuroinflammation
50 mcg per spray, 4-8 sprays daily (CIRS/nasal); 50-150 pmol/kg/min (IV infusion)intranasal or oral
Frequency
not stated
Reconstitution
Sterile 0.9% saline
Reported by
Peptide Protocol Wiki
What it saysHide(secondary reference; provider confirmation required).
Standard: 100 mcg · Low: 50 mcg · High: 200 mcgSubQ
Frequency
1-2x daily
Duration
Ongoing
Cycle
12 week cycles.
Reconstitution
A 10 mg subcutaneous vial with 3 mL bacteriostatic water gives about 3.33 mg/mL.
Reported by
Peptide Dosing Protocols
What it saysHide(secondary reference; provider confirmation required). Half-life: very short. The standard dosing for VIP is 100 mcg taken 1-2 times daily.
50 mcg per spray intranasally, up to 4 times daily (titrate per CIRS protocol; typically after correcting other abnormalities)intranasal
Frequency
up to 4 times daily
Duration
Per provider direction
Vial
Compounded intranasal spray (e.g. 50 mcg/actuation)
Reconstitution
Compounded intranasal spray - no reconstitution required
Reported by
Commonly published practitioner dosing range - provider confirmation required.
Vladonix
Recovery
1-2 capsules daily (commonly 2 capsules daily)oral
Frequency
once or twice daily
Duration
20-30 day course
Cycle
Repeat course 1-2x per year
Vial
Oral capsules (peptide bioregulator)
Reconstitution
Not applicable (oral capsule)
Reported by
Commonly published practitioner dosing range - provider confirmation required.
Wolverine
Tissue Repair · Soft Tissue Recovery · Injury Recovery · Recovery
Pre-blended: 0.5-2.0 mg total blend daily (0.25-1.0 mg BPC-157 + 0.25-1.0 mg TB-500); Separate vials: BPC-157 250-500 mcg daily, TB-500 2.0-5.0 mg 2x/week (loading), TB-500 2.0 mg 1-2x/week (maintenance)subcutaneous
Frequency
Pre-blended: daily; Separate vials: BPC-157 daily, TB-500 2x/week (loading) or 1-2x/week (maintenance)
Duration
6-12 weeks total; loading phase weeks 1-4, maintenance phase weeks 5-8 or 5-12 depending on protocol; off-cycle gaps of 2-4 weeks commonly described
Reconstitution
Pre-blended 20 mg vial: Add 2.0 mL bacteriostatic water → 10 mg/mL total (5 mg/mL each compound); 10 units (0.10 mL) delivers 0.5 mg BPC-157 + 0.5 mg TB-500; ~20 doses per vial at 1.0 mg/day. Separate vials: Add 2.0 mL BAC water to each 10 mg vial → 5.0 mg/mL each. Steps: allow lyophilized peptide to reach room temperature; wipe both vial stoppers with alcohol swabs; draw planned BAC water volume into sterile syringe; inject BAC water against inside wall of peptide vial slowly, never directly onto powder; swirl gently until fully dissolved, do not shake; verify solution is clear (TB-500 may appear very faintly tinted); refrigerate at 35.6-46.4°F (2-8°C) and use within supplier's stated beyond-use window.
Reported by
peptidedosingprotocols.com
What it saysHide(secondary reference; provider confirmation required). [Source: peptidedosingprotocols.com]
1.0 mg total blend daily (0.5 mg of each compound) from a pre-blended 20 mg vial.
Frequency
not stated
Reconstitution
Reconstitute with 2.0 mL BAC water for a final concentration of 10 mg/mL total blend (5 mg/mL each component).
Reported by
Peptide Dosing Protocols stack
What it saysHide(secondary reference; provider confirmation required).
Reconstitution: before you begin
  • Reconstitute with bacteriostatic water, added slowly against the vial wall. Never shake.
  • Draw measured units on a U-100 31G 1 mL / 5⁄16″ (8 mm) insulin syringe.
  • Store reconstituted vials refrigerated, and observe each compound's own stability window.
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