Humanin
Humanin is a small mitochondrial-derived peptide studied for its role in cell survival, mitochondrial function, and aging biology.
We have not published an audit on Humanin. Everything below is reference material, not our own reading of the literature, treat it as a starting point for questions, not as a grade.
How it is thought to work
Humanin is encoded inside mitochondrial DNA and is involved in cytoprotection and neuroprotection. It has been shown to have effects on aging and cardiac health in animal studies. [Source: Peptide Dosing Protocols]
Humanin is a rare peptide made by mitochondria that helps protect cells from dying by blocking a key cell-death protein and activating survival signals; its lab-made version HNG works the same way but is more stable and potent.
Reported side effects
The most commonly reported effects are injection-site redness or discomfort, mild fatigue, occasional headache, and transient nausea at higher doses, though all of this comes from anecdotal community reports rather than human clinical trials.
Who should avoid it
People with active or recent cancer, pregnancy, children, those on insulin or blood-sugar-lowering drugs, those with active autoimmune or inflammatory disease, and anyone without access to qualified medical oversight or sterile injection conditions should avoid humanin and HNG.
What sources report
These are figures published elsewhere, reproduced with their source. Peppersite does not recommend an amount for Humanin or for anything else, and nothing below has been checked against the study it came from.
| Amount | Frequency | Route | Reported by |
|---|---|---|---|
| Standard: 4 mg/kg · Low: 2.5 mg/kg/injection | 2x/week4-12 weeks · 6-8 weeks | IP | Peptide Dosing ProtocolsWhat it saysHide(secondary reference; provider confirmation required). The standard dosing for Humanin is 4 mg/kg administered via intraperitoneal injection twice weekly. |
- 2x/week4-12 weeks · 6-8 weeks
- IP
- Peptide Dosing Protocols
What it saysHide
(secondary reference; provider confirmation required). The standard dosing for Humanin is 4 mg/kg administered via intraperitoneal injection twice weekly.
- reconstituted with 2 mL bacteriostatic water (10 mg/mL)
Common questions
What is humanin?
Humanin is a small mitochondrial-derived peptide encoded inside mitochondrial DNA, discovered in 2001 as a factor that protected neurons from amyloid-beta toxicity.
What is HNG?
HNG is a synthetic analog of humanin that is more stable and significantly more potent than native humanin.
How is humanin commonly dosed in research planning?
The most-cited protocol uses HNG 4 mg/kg intraperitoneal twice weekly in middle-aged mice.
How is humanin reconstituted?
Humanin and HNG are reconstituted by adding 2 mL of bacteriostatic water to a 20 mg vial.
Check 20 mg in 2 mL in the calculator →What is the half-life of humanin?
Native humanin has a short circulating half-life of about 30 minutes.
What route is humanin given?
Animal studies have most often used intraperitoneal injection, while research planning typically describes subcutaneous injection.
How long is a typical humanin cycle?
Research planning typically describes cycles of 4 to 12 weeks.
What supplies are needed for a humanin protocol?
A typical setup includes the humanin or HNG vial, bacteriostatic water, U-100 insulin syringes, alcohol prep swabs, and a sharps container.
Sources
The reference sections above are imported from our compound knowledge base, which classes the evidence for Humanin as emerging. That classification is the knowledge base’s, not ours, our own grade appears only where an audit is linked above. Research use only; nothing here is medical advice or a protocol.
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